Automated MAb Workflow: from Harvest Cell Culture to Intact Mass Analysis of Variants
16 Dec 2014
During development of recombinant MAbs, a large number of harvest cell culture (HCC) samples must be screened for IgG titer, aggregations, and charge variants. This application note demonstrates how automated analysis can be achieved with the Thermo Scientific Dionex UltiMate 3000 x2 Dual Titanium Biocompatible Analytical LC System. This workflow enables the completion of affinity purification, size-exclusion analysis, and charge variant analysis in less than one hour.