Application of the Profinity eXact™ Fusion-Tag System to Eukaryotic Expression Systems
13 April 2015
Protein overexpression and affinity purification are used in various biological research fields and play an important role in the postgenomic era. This application note demonstrates small-scale transfection of insect Sf9 cells and mammalian HeLa cells followed by expression and purification analysis. The results indicate that the Profinity eXact system can be used in eukaryotic cells for single-step purification of tag-free proteins with low background and without compromising the obvious function of the system.