Automation of CE-SDS Sample Preparation for PA 800 plus IgG Purity/Heterogeneity Assays Using a Biomek 4000 Automation Workstation
11 Nov 2013

This application note demonstrates the automation of a CE-SDS workflow, to prepare IgG protein samples for the Beckman Coulter PA 800 plus Pharmaceutical Analysis System, on the Biomek 4000 Workstation. The fully automated denaturation and reduction of IgG controls resulted in highly reproducible peak migration times with CVs <2% and corrected peak areas with CVs <3% using UV absorbance at 220nm. These results were accomplished using both β-mercaptoethanol (n=48) and TCEP (n=24) as reducing agents and demonstrated no cross contamination between samples.

Show    per page
1 of 2     Page  1 2 Next 
Automation of CE-SDS Sample Preparation for PA 800 plus IgG Purity/Heterogeneity Assays Using a Biomek 4000 Automation Workstation protein analysis, molecular weight analysis30 Oct 2013Read
High Throughput TruSeq Stranded mRNA Library Construction on the Biomek FXP library preparation, NGS, RNA23 Oct 2013Read
Automated TruSeq DNA Sample Preparation using Biomek FXP Liquid Handler DNA, library preparation, NGS23 Oct 2013Read
Reproducible and Efficient Automation of PCR Reaction Setup and Purification Using the Biomek 4000 Workstation and AMPure XP qPCR, PCR purification22 Oct 2013Read
Automation of PCR Reaction Setup Application Using the Biomek 4000 Laboratory Automation Workstation qPCR, PCR automation22 Oct 2013Read
Biomek Automated 384-well qPCR Setup using KAPA Biosystems Library Quantification Kit for Illumina Sequencing Platforms qPCR, PCR automation22 Oct 2013Read
Automated TruSeq RNA Sample Preparation from FFPE Tissue Specimens utilizing the Biomek FXP Liquid Handler tissue processing, DNA fragment sizing, library preparation22 Oct 2013Read
Highly Efficient MicroRNA and Total RNA Purification from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) size selection, miRNA, FFPE extraction13 Sep 2013Read
CESI-MS: An Essential Complement to nanoLC-ESI-MS for Complex Proteome Analysis protein analysis, protein quantitation, peptides25 Jul 2013Read
Gel-Free Size Selection Using SPRIselect For Next Generation Sequencing size selection, DNA fragment sizing, cancer26 Jun 2013Read

Popular tags in SelectScience

Buying guides

Write a Review

Join the Global SelectScience Community Today! It’s FREE!

Helping you make informed decisions about the latest lab products and technologies:

  • Be the first to learn about new technologies
  • 10,000+ trusted product reviews from your peers
  • Compare 500,000 products and manufacturers
  • Get exclusive buying tips from experts
  • Find solutions fast: 10,000+ Application Notes, Webinars and Videos

Connect with 250,000 scientists... All in one place!

Become a member today!