Megaruptor® Genomic DNA Shearing for Large Insert Sequencing Library Preparation
6 January 2016
This application note describes the use of the Diagenode Megaruptor® for optimizing the library preparation procedure, targeting very large DNA molecules used for Pacbio sequencing, of Wageningen University. The rapid developments of this third generation sequencing resulting in continuously increased read length requires a reliable DNA fragmentation into large molecules. The benefit of these large DNA molecules is their ability to span repetitive sequences which appeared to be crucial for de-novo reconstruction of large and complex genomes. Using large hydropores, they were able to evaluate target fragmentation size, fragment range, reproducibility and the ease in usage. The Megaruptor® proved to be intuitive, easy to use and has a broad range of DNA fragmentation applications.