Fluorescence Quenching in Cell-Based Assays Can Be Dye Specific: A Case Study Using Both Fura-2 AM and Fluo-4 AM Calcium Reporter Dyes Using the FDSS6000
15 Dec 2014

In this case study we screened a panel of 3500 compounds for antagonism using both Fura-2 AM and Fluo-4 AM dye loaded cells. Results reveal some compounds mediate strong inhibition, reported as no change in fluorescence upon agonist addition, against one dye-loaded cell model and not the other, suggesting dye-specific quenching.

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