Detection of Human Tau Protein Aggregation
7 October 2015
The tau protein stabilizes microtubule structures in the brain. These structures are supporting the nutrient transport between neurons. Abnormal tau protein leads to collapse of structure and transport – plaques will be developed. This happens in patients that undergo neurodegeneration, e.g. in Alzheimer´s. The level of a patient´s tau protein can therefore be an indicator of a neurodegeneration disease state. To that end Cisbio developed a tau aggregation kit that can be applied to cell cultures, brain tissue extracts, and recombinant proteins. This application note demonstrates the measurement of tau aggregates using a sandwich immunoassay, applying an anti-tau monoclonal antibody labeled either with terbium-cryptate or d2, ensuring assay quality reproducibility and signal quality.