Enhancing Field of View Through Image Montaging and Stitching
26 Jun 2014
Widefield microscopy can provide the spatial resolution to view individual cell processes, but there is a limitation to the field of view, which is inversely proportional to the magnification provided by the microscope objective used. This can be problematic when tissue slices need to be imaged. However, it is possible to automate image capture resulting in a montage of images that can then be used to reconstruct an image of the entire object by stitching images. This application note demonstrates the ability to capture a series of images that can then be used to reconstruct the entire cross-section of a mouse kidney.