Detection of Human IgG Using a Luminescence-based Proximity Assay
19 Dec 2014

This application note describes the use of the Synergy Neo Multi-Mode Microplate Reader in conjunction with the Spatial Proximity Analyte Reagent Capture Luminescence (SPARCL) assay technology to quantitate human IgG. (SPARCL) technology is a proximity dependent, chemiluminescent detection technique. In the assay, a chemiluminescent substrate (acridan) is brought into the proximity of an oxidative enzyme (horseradish peroxidase, HRP) through the specific antigen/antibody interaction. Once in close proximity adding the “trigger” reagent results in a flash luminescent signal that is proportional to the amount of analyte.

Show    per page
1 of 8     Page  1 2 3  ... Next 
Cell Synchrony Determination Using Microscopic Imaging cell imaging, cell cycle analysis, cell proliferation8 Dec 2014Read
Exploring a Simpler Solution for Fluorescence Imaging cell imaging, fluorescence microscopy4 Dec 2014Read
A Label-Free, Multi-Omic Study to Qualitatively and Quantitatively Characterize the Effects of a Glucosylceramide Inhibitor on Obesity chromatography method development4 Nov 2014Read
High throughput Miniaturization of Cytochrome P450 Inhibition Screening Using the Echo® Liquid Handler ADME, screening4 Nov 2014Read
The Impact of a 3-Dimensional Human Liver Microtissue Model on Long-term Hepatotoxicity Studies microtissue, hepatotoxicity4 Nov 2014Read
Good Westerns Gone Bad: Tips to Make Your NIR Western Blot Great24 Oct 2014Read
Human and Rat Neuronal Microtissues for Toxicity Testing 3d cell culture, neurons, microtissue9 Oct 2014Read
Automated Method Development Using the Agilent RapidFire 365 High-Throughput Mass Spectrometry System8 Oct 2014Read
Real Time Calcium Flux Measurements in iPSC 3d cell culture, fluorescence based assay, animal models27 Aug 2014Read
The Use of Radiolabelled Compounds in the Investigation of Metabolism and Drug-Drug Interactions In Vitro ADME, compound screening, Toxicology Screening22 Aug 2014Read

Popular tags in SelectScience


Buying Guides