Understanding Cell Motility: Next-Generation Scratch Wound Healing Assay
27 Feb 2018
The wound healing assay is an established in vitro procedure that measures collective cell migration, and replicates the sheet migration that occurs in a range of biological processes, such as:
- cancer metastasis
- embryonic morphogenesis
- tissue injury
A common limitation of conventional approaches to the wound healing assay is that it can be influenced by both cell migration and cell proliferation. In this webinar, Dr. Rakesh Suman, senior biological application scientist at Phasefocus, will discuss the Livecyte™ system and how it can significantly enhance the conventional wound healing assay and diminish this limitation. Dr. Matt Hardman and postdoctoral researcher, Liz Roberts will also discuss how the Livecyte system has helped propel their research.
By providing a unique, quantitative cell analysis system which delivers continuous tracking and analysis of individual cells within large populations, Livecyte enables users to gain a more realistic and comprehensive understanding of cell behavior. The high-contrast, label-free images produced enable multi-parametric analysis to be performed at an individual cell level, with minimal cell perturbation. This single-cell analysis directly measures cell migration, whilst also providing insights to the morphological phenotype during the wound healing process.
This webinar will explore:
- Advantages of continuous monitoring of the wound healing process using label-free Quantitative Phase Imaging (QPI) imaging
- How to separate cell motility from cell proliferation
- The ability to segment and track individual cells along the leading edge to directly measure cell motility
- Characterization of morphological and dynamic cell phenotypes during wound healing