A new approach for DNA/RNA sample assessment using the NanoDrop One
26 Jul 2018

Experimental techniques such as PCR, qPCR, cloning, sequencing and transfection require samples of known concentration and purity. The success of an experiment depends in part on the accurate evaluation of the nucleic acid sample. Absorbance at 260nm is the most popular method for DNA and RNA quantification. However, residual chemicals from the nucleic acid extraction process as well as co-purified cell components can affect the A260 concentration result and the purity ratios (260/280, 260/230) don’t tell the whole story about sample purity. Thermo Scientific™ NanoDrop™ One™ Acclaro Sample Intelligence technology uses powerful mathematical algorithms to identify contaminants and deliver accurate information about both concentration and purity for success in downstream experiments.

Webinar attendees will learn:

  1. how contaminants in DNA and RNA samples can affect absorbance at 260 and concentration results
  2. how the purity ratios 260/280 and 260/230, although useful, don’t tell the whole story about sample purity
  3. how the cutting-edge NanoDrop One Acclaro Sample Intelligence technology uses powerful chemometric algorithms to identify contaminants and provide corrected concentrations

Who should attend:
  • Any scientist who works with DNA and RNA samples.
  • Any scientist working with molecular biology techniques such as PCR, qPCR, cloning, sequencing, and transfection.

Thermo Fisher Scientific