Products & Reviews

Digitonin can be used in cell viability and cytotoxicity assays. It is a detergent solution useful for permeabilizing cells and for creating a cytotoxicity chemistry positive control. Supplied at 20mg/ml in DMSO

ATP Assay for Microbiological Analysis of Water

Extract high-quality nucleic acids with less hands-on time.

Rapid detection of firefly luciferase activity with amazing sensitivity and dynamic range.

The Caspase-Glo ® 3/7 Assay System is  a highly sensitive caspase activity assay for use in plate-reader assays.

Optimized PCR master mix: Just add template, primers and water

Pfu DNA Polymerase is a high-fidelity, thermostable enzyme of approximately 90kDa isolated from Pyrococcus furiosus. The enzyme replicates DNA at 75°C, catalyzing the polymerization of nucleotides in the 5´→3´ direction in the presence of magnesium. Pfu DNA Polymerase also possesses 3´→5´ exonuclease (proofreading) activity. Base misincorporations that may occur during polymerization are rapidly excised by this proofreading ac…

New Site-Specific Endoprotease that Targets Proline and Alanine

MOA-based functional bioassay to measure Fc effector activity.

A best-in-class DNA transfection reagent for your research.

Stable at Room Temperature and Easy to Use

The CellTiter 96® AQueous One Solution Cell Proliferation Assay is a colorimetric method for determining the number of viable cells in proliferation, cytotoxicity or chemosensitivity assays. The CellTiter 96® AQueous One Solution Reagent contains a tetrazolium compound [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; MTS(a)] and an electron coupling reagent (phenazine ethos…

The pGEM®-T Easy Vector Systems are convienent systems to clone PCR products.   They offer all of the advantages of the pGEM®-T Vector Systems with added convieneice of recognition sites for EcoRI and NotI flankin the insertion site. Thus, several options exist to remove the desired insert DNA with a single restriction digestion. The pGEM(R)-T Easy Vectr System II contains JM109 Competent Cells in addition to all pf the…

GoTaq®2-Step RT-qPCR System is a reagent system for quantitative analysis of RNA using a two-step reverse transcription-quantitative PCR (RT-qPCR) method. The first step consists of cDNA synthesis using the GoScript™ Reverse Transcription System. Optimized reagents and ultra-active, GoScript™ Reverse Transcriptase result in high-efficiency, full-length cDNA synthesis from rare and abundant targets with up to 5 µg of RNA inp…

GoTaq® Hot Start Polymerase contains the high-performance GoTaq® DNA Polymerase bound to a proprietary antibody that blocks polymerase activity. The system is supplied with a tube of 25mM MgCl2, allowing optimization of the magnesium concentration in your reactions. It is also supplied with 5X Green GoTaq® Flexi Buffer and 5X Colorless GoTaq® Flexi Buffer. The buffers contain a compound that increases sample density, so tha…

GoTaq® Hot Start Master Mixes are premixed, ready-to-use solutions containing GoTaq® Hot Start Polymerase, magnesium, dNTPs and buffer. Reactions can be set up in less than a minute at room temperature; simply add your template, water and primers. Available with either green or colorless reaction buffers, which also serve as loading buffers, allowing you to go directly from thermal cycler to gel analysis. GoTaq® Hot Start Mast…

The GSH-Glo™ Glutathione Assay is a luminescence-based assay for the detection and quantification of glutathione (GSH). The assay is based on the conversion of a luciferin derivative into luciferin in the presence of glutathione, catalyzed by glutathione S-transferase (GST). The signal generated in a coupled reaction with firefly luciferase is proportional to the amount of glutathione present in the sample. Luminescence is gen…

The CellTox™ Green Cytotoxicity Assay measures changes in membrane integrity that occur as a result of cell death. The assay is intended to assess cytotoxicity in cell culture after experimental manipulation. The assay system uses a proprietry asymmetric cyanine dye that is excluded from viable cells but preferentially stains the DNA from dead cells. When the dye binds DNA released from cells, its fluorescence properties are s…

The QuantiFluor® dsDNA System contains a fluorescent DNA-binding dye that enables sensitive quantitation of small amounts of double-stranded DNA (dsDNA) in solution.   The quantitation of dsDNA is a very important step in many biological applications, particularly in standard molecular biology techniques. The dye shows minimal binding to single stranded DNA (ssDNA) and RNA, allowing specific quantitation of dsDNA. The…

Recombinant RNasin® Ribonuclease Inhibitor reliably protects your RNA samples from RNase degradation.   RNases are ubiquitous and degradation can cause significant damage to RNA, hindering downstream reactions. Protect your sample with the most robust inhibitor available-- Recombinant RNasin® Ribonuclease Inhibitor. Recombinant RNasin Ribonuclease Inhibitor Features & Benefits:   Inhibits a broad spectrum o…

The resin-based Wizard® Plus Minipreps DNA Purification System provides a simple and reliable method for rapid isolation of plasmid DNA.   When using the standard protocol, the entire miniprep process can be completed in 15 minutes or less, with no organic extractions or ethanol precipitations. Minipreps may be processed individually or in multiples with the Vac-Man® (20-sample capacity, Cat.# A7231) or Vac-Man® Jr. (2-s…

The CellTiter-Glo® 3D Cell Viability Assay is a homogeneous method to determine the number of viable cells in 3D cell culture based on quantitation of the ATP present, which is a marker for the presence of metabolically active cells.   This ready-to-use reagent is based on the original CellTiter-Glo® Luminescent Cell Viability Assay chemistry and eliminates the need to combine buffer with lyophilized substrate when prepa…

The PureYield™ Plasmid Maxiprep System isolates transfection-quality plasmid DNA. The system provides a rapid method for purification of up to 1mg of plasmid DNA from a 250ml bacterial culture. Plasmid DNA can be purified rapidly with the vacuum protocol, greatly reducing the time spent on purification compared to silica resin or other membrane-column methods. The PureYield™ Plasmid Maxiprep System incorporates a unique End…

FuGENE® HD Transfection Reagent is a novel, nonliposomal formulation designed to transfect DNA into a wide variety of cell lines with high efficiency and low toxicity. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagent/DNA complex. Additionally, the FuGENE® HD Transfection Reagent has been shown to support transfection in che…

The NanoBRET™ Target Engagement (TE) Intracellular BET BRD Assay measures compound binding at select BET bromodomain target proteins within intact cells. This target engagement assay is based on the NanoBRET™ System, an energy transfer technique designed to measure molecular proximity in living cells