Member since: 2019
Organization: T.E.N. Healthcare LLC
Application Area: Extraction of nucleic acids from yeast
"Good enzyme and clear instruction for making enzyme to use."
Member since: 2016
Organization: Trinity College of Dublin
Exceptional results for NGS either DNAseq, RNAseq or methilomes!
Application Area:Genomic analysis
"Is the best enzyme to obtain high quality gDNA for NGS high fidelity sequencing!"
Member since: 2017
Organization: Millipore Sigma
Great product. If you need zymolyase, buy this one.
Application Area:Purification of yeast genomic DNA
"Bought the product and accidentally reconstituted the whole vial. When used right away, it showed fantastic results. When used over a month after reconstitution and 4°C storage, it still showed activity, just had to incubate longer and add more. Great product. Strongly recommend."
Zymolyase®, produced by a submerged culture of Arthrobacter luteus, has strong lytic activity against living yeast cell walls, to produce protoplast or spheroplast of various strains of yeast cells. An essential enzyme for the lytic activity of Zymolyase® is Beta-1,3-glucan laminaripentaohydrolase. It hydrolyzes linear glucose polymers at Beta-1,3-linkages and releases specifically laminaripentaose as the main and minimum product unit.
There are two preparations of Zymolyase®, Zymolyase®-20T and Zymolyase®-100T, having lytic activity of 20,000 units/g and 100,000 units/g respectively. Zymolyase®-20T is ammonium sulfate precipitate while Zymolyase®-100T is a further purified preparation by affinity chromatography. Lytic activity varies depending on yeast strain, growth stage of yeast, or cultural conditions.