Seahorse XFe Analyzers by Agilent Technologies

4.6
/
5.0
  |  12 reviews
XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.


Seahorse XFe Analyzers by Agilent Technologies product image
Seahorse XFe Analyzers
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Average Rating: 4.6
12 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2020

  • Organization: University Of Salamanca



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great technology! It changed my life
Rating: 5.0

  • Application Area: Cell biology

"It is a game-changer in your metabolism assays, it helps you to better understand how mitochondria are working in your cells or tissues."

Review date: 09 Feb 2021 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: MD Anderson



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great way to see the effect on cell faith and recovery after treatments.
Rating: 4.0

  • Application Area:Mitochondrial research

"Cellular metabolism is a critical factor in stem cell research with recent reports suggesting that both metabolic status and cell culture conditions can significantly alter the differentiation trajectory of stem cells, as well the effect of drugs like chemotherapy and the recovery of cells with stem cells treatment."

Review date: 09 Feb 2021 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: JMI



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Must for cancer metabolism research.
Rating: 4.7

  • Application Area:Metabolism

"Must for metabolism experiment with clarity in real time."

Review date: 02 Feb 2021 | Seahorse XFe Analyzers
  • Status:

    Advanced Reviewer

  • Member since: 2018

  • Organization: University of Illinois at Urbana Champaign



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great and precision results. A key to success for drug discovery.
Rating: 5.0

  • Application Area:Cell metabolism studies

"There are different models of the Agilent Seahorse available on the market, among them I found the best one is the Agilent Seahorse XFe96 Analyzer because the user can use 96 well plate means to measure the 96 samples in one run. We use the Agilent Seahorse XFe96 to measure the oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a 96-well plate format. The oxygen consumption rate (OCR) and extracellular acidification rates (ECAR) are key indicators of mitochondrial respiration and glycolysis as well as the ATP production rate. This measurement together provides a systems-level view of cellular metabolic function in cultured cells and in vitro samples. The story is not over here, one more powerful software tool is also available in the market like MITOsym from simulation plus. MITOsym uses for the mathematical modeling of the data obtained from the Seahorse XFe analyzer for cellular respiration and bioenergetics. The results obtained from the Seahorse XFe analyzer is very reliable and useful for the development and discovery of new drugs for cancer."

Review date: 14 Nov 2020 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Eastern Finland



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Intuitive instrument for live cell analysis.
Rating: 4.3

  • Application Area:Live cell analysis of mitochondrial function

"The Seahorse XFe96 cell analyzer is a benchtop instrument that is mainly intended for our group to analyze mitochondrial function in live cells. It is quite intuitive and the user interface is easy to use with simple directions to operate the analyzer. The data generated is medium throughput and will be adequate for a research group. However, given the cost of consumables from Agilent, researchers may be tempted to use certain reagents sold by other manufacturers."

Review date: 16 Mar 2020 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Smurfit Institute of Genetics, Trinity College Dublin



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Fantastic method for examining mitochondrial health, but costs can add up quickly!
Rating: 4.0

  • Application Area:Analysing cell lines for mitochondrial deficits

"The Seahorse XFe96 is certainly the most powerful and effective tool I have used to measure oxygen consumption in my target cell populations. The plate format is a revelation compared to using the dual chamber Oxygraph (admittedly the Oxygraph has higher resolution, but the ability to examine 96 wells simultaneously is a real boon). The assays themselves are relatively straightforward to set up, but in my opinion, the software causes it to lose a star for Ease of Use - it is very slow to perform simple operations and this is even on the computer supplied with the machine. Furthermore, the data analyzer plug-ins can be very restrictive in what they allow you to do, such as defining where your acute injection was added in the scheme of the assay. All interactions I have had with technicians when looking for advice on assay set up etc has been top notch, they have been professional and helpful, so 5 stars for that. However, it is a very expensive machine to purchase and the consumables are no cheaper. It is possible of course to buy reagents from 3rd party retailers, but without the guarantee of them working, it can cost more in the long run. When you have a run that works well it is second to none in the amount of information you can gain, but when lengthy optimization processes are needed the costs can add up very quickly."

Review date: 06 Mar 2020 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Southern California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use and good results.
Rating: 5.0

  • Application Area:Analyzing metabolic markers in cells

"Good data with the software - makes good graphic presentations."

Review date: 26 Nov 2019 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Humber College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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An important instrument for any lab that measures metabolism.
Rating: 5.0

  • Application Area:Measure rates of glycolysis and oxygen consumption in isolated cells.

"The company provides you with excellent, thorough training and protocols before you start any experiments. As long as you follow the steps, the assay will work. Results are easily analyzed and quite reproducible. A very sensitive and effective tool to measure rates of glycolysis and oxygen consumption that you can monitor in real time."

Review date: 06 Nov 2017 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: St. Jude



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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It is great tool to check metabolism in the cells
Rating: 4.3

  • Application Area:stem cell metabolism

"It is not simple and cheap for all the users. But Seahorse also provide all the great consumables (plates, inhibitors, buffers) so it is really helpful for your experiment even you are the first user in your school/department."

Review date: 25 Jan 2017 | Seahorse XFe Analyzers
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Cleveland State University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great results
Rating: 4.7

  • Application Area:Tumor energy metabolism

"Very sensitive data acquisition and processing. minimal error and excellent data "

Review date: 19 May 2016 | Seahorse XFe Analyzers


  • Status:

    Reviewer

  • Member since: 2009

  • Organization: IPAC RAS



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Now Seahorse is the most useful instruments in our lab!
Rating: 4.7

  • Application Area:Analyse mitochondrial and cellular respiratory capacity, screening of mitoactive compounds

"Allow to provide significantly more experiments simultaneously, this is very important for isolated mitochondria as for screening potential drugs, as for analysing the bioenergetic pattern for brain mito after in vivo experiments "

Review date: 22 Jan 2016 | Seahorse XFe Analyzers
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: Howard Hughes Medical Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Rating: 5.0

  • Application Area:Cells

"It is so reliable that other labs in the institute are coming over to use it too."

Review date: 25 Mar 2015 | Seahorse XFe Analyzers

XFe Extracellular Flux Analyzers simultaneously interrogate the two major energy producing pathways of the cell – mitochondrial respiration and glycolysis - in a microplate, in real-time.  

The measurement of cellular bioenergetics on live cells enables time-resolved analysis and testing of multiple conditions per assay well. XF assays provide increased throughput and use less sample compared to conventional respirometry techniques.  XFe Analyzers are available in two formats- 96-well for maximum throughput and flexibility, and 24-well for specialty samples such as islets and small model organisms.        

Features:  

  • Live-Cell, Real-Time Assays
  • Detect responses to substrates, inhibitors, and other compounds in real time via the 4-port injection system and automated mixing
  • Direct Measure of Energy Metabolism
  • Validate results from protein/gene expression experiments with a functional assay
  • Label-free platform
  • No staining, analyte extraction, or destructive sample processing required
  • Minimal Sample Required 
  • As few as 5000 cells/well (varies by cell type and instrument)

Product Overview

Seahorse XFe Analyzers by Agilent Technologies product image

Seahorse XFe Analyzers

Manufacturer Agilent Technologies

4.6 / 5.0 | 12 reviews