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Trypsin/Lys-C Mix, Mass Spec Grade by Promega Corp.

Manufacturer Promega Corp.
5.0
/
5.0
  |  1 reviews
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution. Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix&nbs... Read more


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Trypsin/Lys-C Mix, Mass Spec Grade
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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money
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  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Allergan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Always works perfectly. Never fails and is never contaminated.
Rating: 5.0

  • Application Area: Peptide mapping

"In our lab we only use Promega Trypson/Lys-C. We've been using these reagents for over 9 years without ever having an issue attributable to the reagent. Not the same with all other protease suppliers."

Review date: 27 Jun 2019 | Trypsin/Lys-C Mix, Mass Spec Grade
Trypsin/Lys-C Mix, Mass Spec Grade, is a mixture of Trypsin Gold, Mass Spectrometry Grade, and rLys-C, Mass Spec Grade. The Trypsin/Lys-C Mix is designed to improve digestion of proteins or protein mixtures in solution.

Using the conventional trypsin digestion protocol (i.e., overnight incubation at nondenaturing conditions), Trypsin/Lys-C Mix improves protein digestion by eliminating the majority of missed cleavages, which occur at prominent quantities in trypsin digests. Trypsin/Lys-C Mix enhances digestion and compensates for the trypsin proteolytic inefficiency at lysine sites.

Trypsin/Lys-C Mix, Mass Spec Grade Features & Benefits:

  • Simple to Use - Use standard overnight digestion with nondenaturing conditions.
  • Enhanced Proteolysis - Increase peptide recovery, resulting in enhanced protein quantitation and improved reproducibility and eliminating the majority of missed cleavages (Figures 1 and 2).
  • Tolerant to Trypsin-Inhibiting Contaminants - Generate mass spectrometry data from poor-quality sample material.