TrueGel3D® HTS Hydrogel Plates for 3D Cell Culture by Merck

The TrueGel3D^® HTS Hydrogel Plate is a ready-to-use solution for easily establishing 3D cell cultures using fully synthetic, PEG-based hydrogels in a simple and automation-compatible manner. These innovative hydrogels contain gradually increasing crosslinking densities throughout the well. Users can proceed directly with seeding cells without any hydrogel preparation or encapsulation steps. After cell seeding, cells will gradually infiltrate the hydrogel and establish a 3D cell culture environment within days. Additionally, this newly engineered hydrogel surface gives the user the possibility to sequentially establish co-culture systems by seeding different cell populations at different time-points in the same hydrogel well.

Compared to other hydrogels based on biological extracts from animal cells, TrueGel3D™ does not contain any products of animal origin that could interfere with or contaminate experiments. Hydrogel components preserve cell viability and allow critical features of the natural extracellular matrix (ECM) to be replicated to more accurately reflect native cellular environments similar to those of tissues by supporting cell adhesion and migration. TrueGel3D™ technology provides mechanical and biochemical cues to investigate both morphological and physiological properties of cells in the 3D environment. 

Features and benefits:

• No hydrogel assembly step required: The hydrogel plates are ready for cell seeding.
• Fully synthetic: Lot-to-lot consistency and control over culture conditions. No animal derived byproducts.
• Sequential seeding: Easily co-culture various cell populations at different time points.
• Designed for imaging: Glass bottom 96-well black polystyrene plates. Precast hydrogel is optically clear. 
• Easy storage: Plates can be stored at room temperature

Applications:

• 3D cell culture
• Stem cell culture
• Co-culture experiments
• Cell imaging
• Tumor spheroid formation
• Tumor microenvironment studies
• Extracellular matrix deposition
• Immunology, angiogenesis, neuroscience, ADME/toxicology