The Troponin I Rapid Test is a rapid immunoassay for measurement of Cardiac Troponin I (cTnI) in human whole blood, serum and plasma specimen. The Troponin I Rapid Test is an immunchromatography assay for measurement of cTnI in whole blood, serum and plasma. The membrane strip contains one test line and one control line; streptavidin for biotinylated cTnI antibody and rabbit anti-goat IgG antibody for the control line. A dye pad is placed at the end of the membrane containing biotinylated cTnI antibody and gold colloidal particles coupled with cTnI antibody. When a sample is applied into the sample well, the cardiac makers present in the sample bind to the specific antibodies coupled with gold particles. cTnI in a sample binds to both cTnI specific dye coupled antibody and biotinylated antibody. The immune complexes move along the nitrocellulose membrane through the test lines and bind to streptavidin immobilized on the test line. Unbound immune complexes pass through the test line and are captured by goat anti mouse antibody in the control line. If the concentration of this marker in the sample is above the cut-off level, red bands appear at the corresponding test lines and the control line. If the concentration of the marker in the sample is lower than the cut-off level, only the colored control line can be seen in the test window. This colored control band must always appear at the control line position (C) for valid test results. A test result is not valid if the colored control line does not appear in the test window.