Status:
ReviewerMember since: 2017
Organization: AHRI
Excellent for purifying a small number of samples at milligram scale.
Application Area: Protein purification
"Very good kit if you need to purify a small number of samples at milligram scale. Contains 2 columns with top and bottom caps. Wash, elution, and neutralization buffers are included in the box, but if you want to store columns for reuse later, you need to provide sodium azide solution. However if you do antibody purification frequently it will be more economical to buy Protein A/G agarose and fill your own columns."
The Thermo Scientific™ NAb™ Protein A Plus Spin Kits are convenient for rapid, small-scale affinity purification of antibodies from a variety of sample types. Each pre-filled microcentrifuge spin column of the immobilized protein resin enables quick purification of ≥34 mg of human IgG from 0.5-2 mL of serum or other sample. Included in each kit are buffers and a streamlined protocol for purifying at least two antibody samples.
Pierce Protein A Plus Agarose is a versatile, high-performance affinity resin for antibody purification. It consists of purified native Protein A that has been covalently immobilized at high density onto high-quality crosslinked 6% beaded agarose (CL-6B). Among the many available varieties of immobilized Protein A affinity resins, this one provides the most versatile combination of chromatographic features for high yield and high purity purification of whole IgG from mammalian serum samples. The agarose beads have physical and chemical properties suitable for many affinity purification systems.
Manufacturer Thermo Fisher Scientific | Available Worldwide
4.0 / 5.0 | 1 reviews