Member since: 2016
Organization: Novartis Pharma AG
Useful reagent for applications aiming at purifying membrane proteins.
Application Area: Selective labelling of cell surface proteins in cell culture and in animal models
"Sulfo-NHS-LC-Biotin can easily be used to selectively label accessible cell surface proteins in cell cultures. Importantly, the cells have to be washed gently but extensively before application of the reagent."
Sulfo-NHS-LC-Biotin is one of three very similar EZ-Link Reagents that are water-soluble, non-cleavable, and enable simple and efficient biotinylation of antibodies, proteins and any other primary amine-containing macromolecules in solution. Specific labeling of cell surface proteins is another common application for these uniquely water-soluble and membrane impermeable reagents. Differing only in their spacer arm lengths, the three Sulfo-NHS-ester reagents offer the possibility of optimizing labeling and detection experiments where steric hindrance of biotin binding is an important factor. Sulfo-NHS-LC-Biotin is offered in several package sizes and as complete protein labeling kits.