Status:
ReviewerMember since: 2017
Organization: Camel Biotechnology Center
Robust easy to use qPCR mix.
Application Area: cDNA synthesis, RT-qPCR
"One step RT-PCR mix, easy to use. No multiple reagents - just a single mix. Add your RNA to the mix. Good for diagnostic RT-qPCR as well as PCR based cloning."
Status:
Advanced ReviewerMember since: 2021
Organization: Ceva Animal Health
Great product for amplification of low-concentrated RNA!
Application Area:Transcribing and amplifying RNA with limited bias
"This product works great for amplifying and transcribing RNA at very low concentrations."
Status:
Expert ReviewerMember since: 2020
Organization: Institute for Biomedic Research of A Coruna (INIBIC)
Reliable.
Application Area:RT-PCR
"Invitrogen™ SuperScript™ VILO™ Master Mix has always given good results, both with genetic material from cell lines and primary cells."
Status:
Expert ReviewerMember since: 2012
Organization: Management of Scientific centers, DPA Al Ain
Robust, easy to use cDNA synthesis mix.
Application Area:cDNA synthesis for PCR or qPCR
"A robust, efficient and easy to use cDNA synthesis master mix. The cDNA components are provided as an easy to use master mix. You just need to add your RNA and water. cDNA synthesis is robust, and complete. Good for cDNA gene cloning or qPCR applications. We have used it to reverse transcribe mRNA, viral RNA for relative gene quantification or molecular diagnostics, Improved sensitivity and ease of use compared to other cDNA synthesis mixes. Although price is higher, the sensitivity and robustness of the mix is excellent. It works with RNA isolated from wide variety of isolation, tolerant to a wide variety of inhibitors."
SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR (qRT-PCR) assays.
For Research Use Only. Not for use in diagnostic procedures.