The SPME-LC fiber probes are intended as single-use devices for the extraction of small molecules out of a fluid followed by solvent desorption and LC analysis.
Traditional solid phase microextraction (SPME) fibers were developed with common GC type phases, which were primarily designed for extraction of volatile or semi-volatile compounds using thermal desorption as the means of introducing sample into the chromatographic column.
The newly developed fiber probes contain C18 silica particles embedded in a proprietary, non-swelling, biocompatible polymer. The benefit of this design enables minimized binding of macromolecules such as proteins and phospholipids, but allows extraction of most smaller analytes of interest.
The fiber core is made from a flexible metal alloy with shape memory properties and a diameter larger than typical SPME fibers. The larger diameter enables the fiber to be used both as the plunger and fiber core, thus simplifying the device construction and reducing the cost of the probe. The fiber is also sealed into a hypodermic needle with an attached hub to allow movement of the fiber for exposure and retraction into the needle.