SmartFlare™ Live Cell RNA Detection by Merck KGaA, Darmstadt, Germany
Status:
Reviewer
Member since: 2017
Organization: University of Liverpool
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- Ease of use
- After sales service
- Value for money
Terrible product, do not use
Application Area: Detection of RNA in cells via fluorescence imaging
"Does not work. Nanoparticles stuck in endosomes, do not enter into cytosol. Scramble control gives false positive."
Review date: 03 Oct 2017 | SmartFlare™ Live Cell RNA Detection
SmartFlare™ RNA Detection Probes use RNA as the analyte and nanoparticle probes for detection. SmartFlare™ probes are designed to fluoresce when they detect specific targets in live cells. By using RNA as the target, SmartFlare™ Probes allow you to identify and analyze various markers in live cells with a single incubation step. After the incubation, the probes exit the cells allowing you to perform downstream analyses with the same, unperturbed cells.
Useful for Stem Cell and Cancer Stem Cell Research
SmartFlare™ RNA Detection Probes have been successfully used in stem cells and cancer stem cells.
The potential for a stem cell to become any cell type yields endless possibilities. However, spontaneous or undesired differentiation is inherent within cell culture conditions. SmartFlare™ Probes can be used to screen and characterize the cells before using them in follow-up experiments.
Identifying cancer stem cells within mixed populations with the odds stacked against you can be challenging. Cancer Stem Cells represent a rare subpopulation of a tumorigenic population. SmartFlare™ RNA detection probes can be used to tag live Cancer Stem Cells regardless of surface protein expression. Probes such as those targeting Nanog have been used successfully to target and isolate Cancer Stem Cells from tumorigenic populations.
Product Overview
