SingleShot™ SYBR® Green One-Step Kit for Cell Lysis and RT-qPCR, 100 x 50 µl reactions by Bio-Rad

Use the SingleShot™ SYBR^® Green One-Step Kit to obtain high-quality RT-qPCR data with SYBR^® Green chemistry directly from cell culture lysates in a one-step workflow 1 hour after cell lysis. All reagents and RNA control included.

Benefits include:

• Cell lysate from 10 to 100,000 cells ready in 20 minutes
• Complete removal of genomic DNA while preserving RNA integrity
• Optimal accuracy and linear dynamic range of qPCR data across genes
• Results comparable to using purified RNA, including dynamic range
• Validated with PrimePCR™ Assays and Panels (visit www.bio-rad.com/primepcr )
• SingleShot RNA Control ensures optimal cell and lysate inputs

Optimized Sample Input

The SingleShot™ SYBR^® Green One-Step Kit maximizes cell lysate input in a one-step RT-qPCR reaction:

Kit Contents

Refer to the user manual for details on reaction setup and troubleshooting tips.

Cell Lines Validated for Use with the SingleShot™ SYBR^® Green One-Step Kit

The SingleShot™ SYBR^® Green One-Step Kit has been validated for use with a number of common human and rodent cell lines, such as all types of adherent and suspension cells, including but not limited to:

Related Products

The SingleShot™ SYBR^® Green One-Step Kit belongs to the family of SingleShot™ Cell Lysis RT-pPCR Kits :

• SingleShot Cell Lysis One-Step RT-qPCR Kits — including SingleShot™ SYBR^® Green One-Step Kit ( 172-5095 ) and SingleShot Probes One-Step Kit ( 172-5070 )
• SingleShot Cell Lysis RT-qPCR Kits — including SingleShot™ SYBR^® Green Kit ( 172-5085 ) and SingleShot Probes Kit ( 172-5090 )
• SingleShot Cell Lysis Kit — available for 100 reactions ( 172-5080 )