Sera-Mag™ Select PCR clean-up and size selection reagent is based on well-known solid phase reversible immobilization technology, for selective binding of DNA fragments for applications such as next generation sequencing (NGS) and PCR clean-up. It combines the convenience of magnetic bead technology, using the exceptional binding characteristics of Sera-Mag Carboxyl Speedbeads with an optimized binding solution in a ready-to-use formulation.
Sera-Mag Select provides a flexible solution for DNA clean-up, recovery and size selection in one ready-to-use solution. By adjusting the amount of reagent that is mixed with a fixed volume of sample, the DNA size range isolated from the process can be adjusted to suit the user’s requirements, from total recovery to narrow range size selection. As a general rule, the smaller the DNA fragment to be bound, the larger the volume of the reagent that should be added (relative to the initial sample volume). DNA of 100 bp size or greater can be reliably recovered using Sera-Mag Select, allowing effective removal of primers, nucleotides and adapters. Different modes of use are described in detailed in the User Guide.
PCR clean-up - Sera-Mag Select can be used to clean up PCR products post-cycling, removing unused oligonucleotides, PCR buffer components and enzymes. By eluting into a smaller buffer volume than that of the input sample, the concentration of the eluted DNA can be increased.