RNA Clean & Concentrator™-25 by Zymo Research

Manufacturer Zymo Research  |   Model: R1017
4.9
/
5.0
  |  24 reviews
The RNA Clean & Concentrator™-25 cleans and concentrates up-concentration at 10 µg of RNA in as little as 5 minutes.


RNA Clean & Concentrator™-25 by Zymo Research product image
RNA Clean & Concentrator-25
Request Pricing

Receive your quote directly from the manufacturer.




Average Rating: 4.9
24 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2022

  • Organization: Miami University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this Review on LinkedinShare this Review on TwitterShare this Review on Facebook
great kit to prepare RNA for RNA seq
Rating: 5.0

  • Application Area: Clean and concentrate total RNA for RNA-sequencing

"Easy and effective kit to inactivate DNA, clean DNases and concentrate total RNA. Using this kit for RNA-seq."

Review date: 09 Jun 2022 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: University of Iowa



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
This kit is a staple in our lab for cleaning up and isolating RNA.
Rating: 5.0

  • Application Area:To clean and concentration RNA samples after basic RNA isolation using Trizol

"We love this product for both cleaning up and concentrating RNA for qRTPCR applications. We like to isolate our RNA with Trizol, but there is sometimes Trizol carry over. This kit always gives us great quality RNA and concentrates is for qRTPCR requirements. This also allows for a DNAse step on the column like other RNA isolation kits. Super easy to use, ordering and getting technical help is always quick, and this kit is comparable in cost with other isolation kits."

Review date: 31 Jan 2022 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2022

  • Organization: Cornell University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Perfect RNA for sequencing
Rating: 5.0

  • Application Area:RNA-Sequencing of plant tissue

"This kit is one of the best I’ve used during my time on the bench. Simple and straight-forward directions, effective packaging, and does exactly what it says it will do. I’ve went through both DNAse and DNAse free clean-ups and each came out perfect when it came time for quality control measures. I trust this kit and will probably use it the rest of my career (along with Zymo’s other products!"

Review date: 25 Jan 2022 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UCLA Neurology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Quick and great yield.
Rating: 5.0

  • Application Area:Brain organoids

"Simple workflow and efficient, highly concentrated final yield."

Review date: 15 Dec 2021 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Veolia Recherche & Innovation



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Very easy to use and good results.
Rating: 5.0

  • Application Area:Environmental microbiology

"We ue this product in order to purify RNA extract from very complex samples such as wastewater ot activated sludge. It is very easy to use and provides good results in terms of PCR inhibitors removal."

Review date: 18 Mar 2021 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: University of Miami



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great price, speedy shipping, and a quality product.
Rating: 4.0

  • Application Area:Clean up RNA

"I honestly was not initially sold on Zymo because, for whatever reason, their Direct-zol columns have never worked for me (RNA is either degraded or simply not there), so I was skeptical, but it turns out that the RNA clean and concentrator kit I purchased was not only cheap, but I got it within three days (unusual these days when everything is on backorder). Most importantly, the results were great. I think part of it has to do with how narrow the silica columns are compared to other kits like Qiagen. This means you can elute in a smaller volume, thereby concentrating the RNA upon desalting it (hence the name). Definitely would recommend for those working on gene expression."

Review date: 17 Mar 2021 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2021

  • Organization: Stanford University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Super easy to use and delivers good results!
Rating: 5.0

  • Application Area:Clean probes for in-situ hybridization

"This product is essential for cleaning up RNA and removing annoying unincorporated UTP that can cause undesired background."

Review date: 16 Mar 2021 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Lucerna, Inc.



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Good and cost-effective RNA purification kit!
Rating: 4.7

  • Application Area:Purification of in vitro transcribed RNA

"Since we routinely need to purify in vitro transcribed RNA, we have tested several RNA purification kits (e.g. Qiagen, Bio-Rad, NEB, etc) in terms of yield, quality, time, and cost. The RNA Clean & Concentrator kit consistently gave us the best yield among all column-based kits. The RNA quality is the same against other kits but it is significantly cheaper than Qiagen and NEB. The protocol has 1-2 steps more than buffer exchange type systems, but the additional time is tolerable. The only problem we have experienced was that this kit is frequently back ordered as of late, probably because of the increase in RNA extraction needs due to COVID-19."

Review date: 04 Dec 2020 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: University of Pittsburgh



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great results! Strongly suggested!
Rating: 5.0

  • Application Area:RNA cleanup for small amount of RNA (less than 5ng and the size above 150 nt)

"We used this product for fragmented RNA cleanup of CLIP-seq and it works very well for small amounts of RNA purification, less than 5 ng. In addition, we also used it to purify the total RNA for total RNA-seq, and 10 microgram of RNA after DNase treatment were flowed for each column. It is very helpful in our rearch."

Review date: 04 Dec 2020 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: UCSD



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Helpful kit for working with RNA.
Rating: 4.7

  • Application Area:Concentrate dilute RNA

"This kit has been very useful when I have an RNA sample that still has contamination in it after my normal extraction which happens sometimes. It is amazingly fast and easy. I have also used this in scenarios where my RNA is too dilute for an experiment and I need it more concentrated. Super easy."

Review date: 05 Jun 2020 | RNA Clean & Concentrator™-25


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Iowa Hospitals and Clinics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great kit - essential for RNA clean up.
Rating: 5.0

  • Application Area:RNA clean up for samples that are low concentration and/or contaminated with guanidinium.

"This kit is highly reproducible and reliable. Whenever I need to clean up samples and remove guanidinium contamination, I use this. Without it, some samples inhibit the reverse transcriptase enzyme, so they will not work in a downstream ddPCR assay. With the Zymo clean up kit, the guanidinium is removed and the RNA sample performs well in the assay."

Review date: 11 Dec 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of California, Los Angeles



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great product, really lowered my background after clean up.
Rating: 5.0

  • Application Area:RNA clean up after RNA labeling

"This is a really nice kit for RNA cleanup. Just follow the manufacture protocol and it will help you get rid of all the small molecules after reaction. Highly recommended product."

Review date: 27 Nov 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UCLA



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Saved me time and did exactly what it was supposed to do.
Rating: 4.7

  • Application Area:Purify RNA

"The RNA Clean and Concentrator columns are well constructed and do exactly what they are supposed to do. I used them in my RT-PCR workflow and saved a lot of time. The only downside is that the volumes I had to use did not fit in the standard microcentrifuge tubes, so I had to split it into multiple and spin the liquid through the columns in more than one go. This is minor though and could potentially be avoided. Will continue using these for post-DNase treated RNA purification."

Review date: 27 Nov 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of the Sciences



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great results and quick, efficient kit.
Rating: 5.0

  • Application Area:Analyze RNA for sequencing

"The product is quick and easy to use with minimal steps but highly effective in purpose. I have been able to highly concentrate low-quantity samples by pooling together. I find it incredibly user-friendly too that I can go straight into using the kit using the aqueous phase of a trizol extraction."

Review date: 27 Nov 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Delhi



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Good products, clean results.
Rating: 5.0

  • Application Area:Transcriptomics

"We use various products of Zymo for RNA work particularly. It gives clean results."

Review date: 07 Jun 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of California-San Francisco



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Essential part of the lab.
Rating: 5.0

  • Application Area:Next generation sequencing

"We use the RNA clean and concentrator kit to purify RNA for RNA-Seq. Its quick and easy to use, in 15-20 min you have your RNA ready to go! We don't have to wait too long for it once we have placed the order and support is always available if we need it. Although the kit is pretty straightforward so we rarely have issues with it."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Harvarv TH Chan School of Public Health



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great product but can be perfected.
Rating: 4.3

  • Application Area:RNA extraction

"Great product, easy to use, smart design of the columns, practical in-column DNase digestion. Now a couple of issues we have encountered: (i) sometimes the matrix of the column falls into pieces, or even goes through the columns, (ii) potentially related to the previous issue, we sometimes have a white precipitate in our purified RNA that is definitely not RNA, (iii) Zymo columns are only compatible with Zymo vacuum which itself is rather poorly designed with 20 slots (why not 24 like most centrifuge, etc?), unnecessary individual valve for each slot and the columns barely stand on the adapters."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: LMU München BMC



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Reliable kit, fast to use.
Rating: 5.0

  • Application Area:RNA clean up

"We have used this kit a lot in our lab for several years already. It always gives good results. RNA is clean, loss of RNA is in an accepatable range. Protocol is very fast."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Uniklinik Freiburg



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Superb results, essential to my work!
Rating: 5.0

  • Application Area:RNA-Protein interactions

"This kit is super easy to use and it saves me al lot of time while working. I've tested a couple of kits and with this one I get the highest yield amount of my RNA and the best quality, I wouldn't go back to any other brand. The delivery time after ordering is very good."

Review date: 05 Jun 2019 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: New York Genome Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great product, would recommend to others.
Rating: 5.0

  • Application Area:Footprinting and sample purification

"It was extremely easy to use and obtained good quality samples afterwards."

Review date: 29 Jul 2016 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Southern California



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Impressive and great results from this kit. Just like what ZYMO promised.
Rating: 5.0

  • Application Area:Human RNA clean up and concentration

"I got very high yields of RNA from this kit. Very impressive kit, easy to handle and quick processing step. I am very excited to get the high quality clean RNA by this kit."

Review date: 29 Jul 2016 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Cantabria University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Easy to use, quickly and good results and good price
Rating: 5.0

  • Application Area:Basic research

"The effectiveness of the product is really good to get good quality RNAs. The results are reproducible and it is very easy to use. The rating of the product is a 5 over 5. And the price is not expensive."

Review date: 29 Jul 2016 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Louisville



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great result. Everybody should try this product.
Rating: 5.0

  • Application Area:For RNA isolation from the cells in culture and gene expression studies.

"I’ve never seen such purity of the RNA before. This product works excellent and easy to use and affordable."

Review date: 28 Jul 2016 | RNA Clean & Concentrator™-25
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Biofidal



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Share this ReviewShare this Review on TwitterShare this Review
Great product!
Rating: 4.3

  • Application Area:RNA clean up before NGS

"The product is very easy to use, very quick protocol for an excellent RNA clean up. The product is also useful: it also purifies small RNA which is not the case of all RNA clean up columns "

Review date: 12 Jul 2016 | RNA Clean & Concentrator™-25

RNA Clean & Concentrator-25 provides a simple and reliable method for the rapid preparation of up to 50 µg of high-quality RT-PCR-ready RNA.  This simple procedure is based on the use of a unique single-buffer system and Zymo-Spin column technology that allows for selective recovery of total RNA (> 17 nt), large RNAs (> 200 nt), and/or small RNAs (17-200 nt).

The procedure is easy:  Add binding buffer and ethanol to your sample, then bind, wash and elute ultra pure RNA.  The RNA can be eluted from the Zymo-Spin IIC Column in as little as ≥25 µl of RNase-free water.  The highly-concentrated, purified RNA is suitable for all subsequent analyses and molecular manipulations.

The entire procedure typically takes about 5 minutes.