Visualize and quantify the total protein content on Western blot membranes with REVERT Total Protein Stain.
REVERT Total Protein Stain provides linear, proportional signal across a broad range of sample concentrations. Use this quick, total protein stain to correct for lane-to-lane variation in loading and transfer of target protein in your quantitative Western blot experiments. Normalizing target protein to a total protein stain like REVERT offers greater quantitative data accuracy. REVERT Total Protein Stain does not covalently modify your target protein and therefore does not affect its quantitation.
Drop REVERT Total Protein Stain right into your existing protocol, without the need for any additional reagents or special gels. The stain will work with the membrane of your choice (PVDF or Nitrocellulose). Stain your blot in just 5 minutes, and image in the 700 nm channel with an Odyssey® Imaging System. For a single-color fluorescent Western, go right to the blocking step, or remove the stain with REVERT Reversal Solution when detecting multiple targets.