ReliaPrep™ miRNA Cell and Tissue Miniprep System by Promega Corp.

ReliaPrep™ miRNA Cell and Tissue Miniprep System provides complete isolation of total RNA, including microRNA (miRNA) and other small non-coding RNA (sncRNA) subspecies, from a wide variety of cell and tissue types as quickly as 40 minutes. The proprietary column/binding matrix can efficiently capture total RNA, including miRNA, from very small amounts of input material. Using this membrane-based purification system, 1 × 102 to 1 × 106 cultured cells or 0.25–20mg of tissue can be processed per purification. The system incorporates a DNase treatment step, which effectively removes substances that can inhibit downstream assays.