The Residual Protein A (RPA) kit is a ready to use setup enabling for sensitive, accurate quantitation of leached Protein A in bioprocess samples while maintaining a simple workflow. The kit utilizes a validated sample treatment method for dissociating Protein A from antibodies that does not require boiling, neutralization or centrifugation steps.
Dip and Read™ RPA biosensors with pre-immobilized chicken anti-Protein A antibody are sequentially dipped into treated samples, buffers, and detection antibody arrayed in 96-well microplates to create a microscale immunoassay on the biosensor tip. Signal detection occurs in real time at the secondary antibody step without need for conjugates or enzymatic reactions.
- Specificity – Designed to detect recombinant Protein A constructs and MabSelect SuRe™
- Sensitivity – Accurate quantitation down to 100 pg/mL recombinant Protein A
- Unattended operation – Automated detection with minimal hands-on time
- Throughput – Analyze 96 samples in under 2 hours on the Octet HTX system
Not intended for diagnostic use.