New recombinant Alkaline Phosphatase
rAPid Alkaline Phosphatase is used to dephosphorylate 5' ends of DNA and RNA. Alkaline phosphatase treatment prevents self-ligation of fragments by removing the 5'-phosphoryl termini required by ligases; this feature is of major importance in cloning strategies to decrease vector background. rAPid Alkaline Phosphatase can also be employed to dephosphorylate proteins.
Use rAPid Alkaline Phosphatase in numerous applications, including: