Plexor® qPCR and qRT-PCR Systems by Promega Corp.

Manufacturer Promega Corp.
The Plexor® qPCR and qRT-PCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry. Each target is measured directly during the amplification process and not through a secondary reaction. Plexor® reactions require only two primers for each target. The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent tag and modified base. As amplification... Read more


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Plexor® qPCR and qRT-PCR Systems
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The Plexor® qPCR and qRT-PCR Systems are multiplex-capable real-time amplification systems using novel base pair chemistry. Each target is measured directly during the amplification process and not through a secondary reaction. Plexor® reactions require only two primers for each target.

The Plexor® Systems work by measuring a reduction in fluorescent signal during amplification. Amplification uses only two primers, one of which contains both a fluorescent tag and modified base. As amplification proceeds, fluorescence is reduced by site-specific incorporation of a fluorescent quencher inserted opposite the complementary modified base. The quencher is in close proximity to a fluorescent dye located on the end of the primer, resulting in a reduction in fluorescent signal.

After PCR, a melt analysis can be performed to provide an internal control for the final assay design or to expedite troubleshooting during development. The system also includes a proprietary reagent to minimize primer-dimer formation.


Plexor® qPCR and qRT-PCR Systems Features & Benefits:

  • Simplify Multiplex Performance - Only two primers are required for each target, making the design of multiplex assays much easier.
  • Improve Productivity - Less labor, less time and less cost per assay. Measure controls and targets at the same time in the same well.
  • Enjoy Convenience - The master mix format provides everything you need in one tube. Combine with your template and primers.
  • Obtain Strong Data - Plexor® Systems measure a reduction in fluorescent signal during amplification. Quenching is directly proportional to amplicon accumulation. After amplification, a melt analysis can be performed to provide an internal control for specificity.
  • Use Your Existing Real-Time Instruments - Plexor® technology works on most currently available real-time instruments capable of measuring more than one fluor. The free Plexor® Analysis Software allows users to import and analyze data from their preferred instrument platform.

Product Overview

Plexor® qPCR and qRT-PCR Systems by Promega Corp. product image

Plexor® qPCR and qRT-PCR Systems

Manufacturer Promega Corp.

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