Rapid, quantitative precipitation of DNA and RNA, including PCR clean-up
Pellet Paint® Co-Precipitant* is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (1, 2). The two minute precipitation requires no low temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even the most dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.
Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated. Pellet Paint Co-Precipitant is ideal for this purpose because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.
Pellet Paint is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although Pellet Paint absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package of Pellet Paint) can be used as a correction factor when determining nucleic acid concentration. Pellet Paint is compatible with Cy5-based automated sequencers. Pellet Paint NF is recommended for use with PE Applied Biosystems automated sequencers.
* patent pending