The pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1 hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.
The pCMV-Script vector is derived from a high-copy-number pUC-based plasmid and is designed to allow protein expression in mammalian systems. Mammalian expression is driven by the human cytomegalovirus (CMV) immediate early promoter to promote constitutive expression of cloned inserts in a wide variety of cell lines. Selection is made possible in bacteria by the kanamycin-resistance gene under control of the prokaryotic ß-lactamase promoter. The neomycin-resistance gene is driven by the SV40 early promoter, which provides stable selection with G418 in mammalian cells.
For Research Use Only. Not for use in diagnostic procedures.