Octet^® R8 BLI System by Sartorius Group

"Its worked well for the 1st year, but there is huge variation in the biosensors. Control reactions won't work for months, even application specialists have no idea what is going on."

"Instrument performance is good. With 8 channels, we are getting results within very short time frames when compared to similar equipment. Software is also very user friendly, all the details are available in a report file. Sensor prices are reasonable and less expensive. No sample loss especially for ordered protein, DNA and other samples. Some sensor type are reusable with protein having histidine tag, GST tag etc.. Small molecules detection is also good for protein-drug, DNA-drug interaction studies. Overall product is good."

"Good and fast for kinetic studies, protein-protein interaction studies, and protein-drug interaction studies."

"Octet is very useful for High Throughput Screening screening of antibodies and mutants. very useful for antibody engineering."

"Very easy and intuitive to use and relatively low cost of biosensors allows pilot work to be undertaken without a huge initial financial commitment, which is important in an academic (i.e. grant-funded) setting. Good choice of biosensors, and is increasing over time, although the manufacturers could provide more details about surface chemistry (e.g. capture sensors that are in fact biotin-streptavidin based). Evaporation on the plate has been addressed by a possible instrument upgrade. Would like to see an option for half-well/lower volume plates. Software upgrades are expensive!"

"Octet Red96 is a very nice piece of equipment. It is great for measuring protein interactions in a high-throughput manner. The affinities obtained are equivalent to what can be measured by other techniques, such as ITC, SPR, MST and others. The equipment can also be used for protein quantification, and can be used in "dirty" samples, such as extracts, as it doesn't use any microfluidics."

"It's an easy going and user friendly instrument. You can get high quality results with lowest amount of protein sample."

"The Octet RED is an excellent instrument for fast quantification and initial screens of antibody binding kinetics. While it may not give the same resolution that is achieved through traditional SPR techniques, the Fortebio Octet RED system is able to produce reliable, accurate and repeatable results at a much higher throughput and a greatly reduced consumables cost. It is absolutely recommended for any application that requires routine kinetics screening of multiple protein binding partners or binding conditions. "

"The system works well."