The NxSeq™ SARS-CoV-2 Whole Genome Library Kit supplies the targeting primers, enzymes and other reagents necessary to build overlapping, targeted amplicon libraries covering the SARS-CoV-2 genome. These libraries enable whole genome viral sequencing on Illumina instruments for the identification and tracking of SARS-CoV-2 variants. We designed the targeting primer pairs against the Wuhan-Hu-1 strain (NC_045512.2) and carefully screened and optimised the primer set to produce high on-target rates and consistent amplicon yields in order to maximise multiplexing, streamline analysis and keep costs low. Each amplicon target region* overlaps with the target regions of adjacent amplicons in order to generate nearly full coverage and effective variant analysis of each SARS-CoV-2 isolate.
Manufacturer LGC Biosearch Technologies | Available Worldwide
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