Methicillin-resistant Staphylococcus aureus (MRSA) is a major pathogen responsible for serious hospital infections worldwide. These bacteria are resistant to all beta-lactam antibiotics due to the production of an additional penicillin binding protein, the PBP2a (about 75kDa) encoded by the mecA gene, which shows low affinity for this class of antibiotics. According to the resistant and sensitive ability of the bacteria to antibiotics, two main groups are classified: MRSA and MSSA (Methicillinsensitive Staphylococcus aureus).
Immunogen was PBP2a recombinant protein derived from MRSA bacteria. This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with PBP2a. The IgG fraction of tissue culture supernatant was purified by Protein G affinity chromatography.
Product is supplied as a powder obtained from lyophilization of purified antibody in PBS without preservatives. Reconstitute the antibody with sterile de-ionized water to a final concentration of 1 mg/ml.
This antibody was selected for its ability to specifically detect PBP2a from MRSA. The antibody has ability to distinguish both MRSA and MSSA in tested assays; in Western Blot analysis, this antibody did not produce a band for any bacterial cell lysates from MSSA. This antibody showed no cross-reactivity with other tested bacterial proteins.
ELISA (recommended work dilution= 1: 80,000); Western Blotting (recommended work dilution= 1:1000) ; (see image) Western Blot: The membrane blot was probed with purified mouse anti-MRSA primary antibody (1:1,000), then with Anti-Mouse IgG secondary antibody conjugated to HRP (1:10,000). The detected protein was clearly visualized by chemiluminescence detection system.
Store at 4°C if intended for use within one month, otherwise, store at -20°C to -80°C. The lyophilized antibody is stable for at least 18 months after the date of receipt when stored at -20°C to -80°C. After reconstitution, it can also be aliquoted and stored frozen at -20°C to -80°C in a manual defrost freezer for 6 months without detectable loss of activity. Upon reconstitution, the antibody can also be stored for 30 days at 4°C. Please avoid freeze-thaw cycles, as this will lower the activity of the antibody.
- Zinderman, C. et al. "Community-Acquired Methicillin-Resistant Staphylococcus aureus Among Military Recruits". Emerging Infect Dis. 2004;10(5):941-4.
- Bignardi GE, et al. Detection of the mecA gene and phenotypic detection of resistance in Staphylococcus aureus isolates with borderline or low level methicillin resistance. J Antimicrobiol Chemother. 1996;37:53–63.