MitoXpress® Intra - [Intracellular] Oxygen Concentration Assay by AMSBIO

MitoXpress®-Intra (NanO2) is a nanoparticulate metalloporphryin-based, phosphorescent, oxygen sensitive probe that is used to monitor intracellular oxygen in populations of live mammalian cells by plate-based, time-resolved fluorometry (TR-F). The assay is based on the ability of O2 to quench the excited state of the probe. The probe is taken up via nonspecific energy dependent endocytosis and, after washing, the cells are monitored on a TR-F plate reader. Probe phosphorescence is quenched by intracellular oxygen in a non-chemical, reversible manner allowing the measurement of average intracellular O2 levels and facilitating real-time monitoring of relative changes in cellular O2 consumption [1-3]. Probe signal increases with a reduction in intracellular oxygen and deceases with an increase in intracellular oxygen.