Member since: 2019
Organization: University of California, Riverside
More than DLS, but beware of a pricey initial investment and unnecessary upkeep costs.
Application Area: Extracellular vesicle analysis
"NTA is a relatively simple technique, but a little expertise is needed to understand how to get good data and reproducible results. Another factor that can be limiting is the small working range, so additional time to get the proper dilution before analysis may be required. The instrument shines at looking at changes between samples and gives a "true" size distribution and particle count, while DLS and other methods use bulk analysis to generate the size distribution and concentration. Regardless of settings, I can measure 100 nm beads as 100 nm +/- 3 nm every time, but the particle count can vary somewhat if the proper care isn't taken in preparing the samples and getting a good video of the sample. The main skill in using this instrument is understanding how to get a good video of your sample, from which the system measures the particles. Another factor can be the material of the particle since some particle will refract light better than others. This can easily be adjusted with a setting, however if you have a high refractive indexed particle mixed with a low refractive indexed particle, you will have issues accurately measuring both. The final consideration I feel is important is that it is difficult to know if your data is correct or not since the method is very non specific. Is that your nanoparticle or some other salt crystal or protein aggregate? The purest water still has artifacts, so really knowing if the particle count and size distribution is correct does require good knowledge of the solution or the sample to know what you are really measuring. Now for the bad. An annoying design flaw is changing from a hard metal plate to plastic consumable top plates on their laser module. They say these newer plastic top plates have better flow characteristics, but it also added a consumable piece that is very fragile. They are sealed with an organic layer, which of course will dissolve away over time making these top plates consumable when the previous design used just a sealing O-ring. It is fairly obvious that this design was chosen to add consumable sales to the instrument so they can continue to get money from the instrument after purchase. Why else would you sell 5 packs of the channel if you did not expect them to wear out quickly? They also are not cheap. The design of the channel does have better flow, but it provides no benefits beyond it takes less volume and you can flush it out with only 1 mL of wash fluid. No benefit is gained in the data besides removing some possible carryover from the previous run if you didn't sufficiently flush the channel. Overall, the instrument has been effective in it's work and we use this instrument fairly heavily, but I did find the after care of the instrument to be incredibly poor. The technicians are knowledgeable, but you will probably need a $12,000 a year service contract to get them to respond. From my experience, every after care call that was done was viewed as a upsales call on their end, with no real care that the instrument is down or that it will cost another large sum of money to get it going again. Even with the service contract, most parts and additional repair costs still are not covered. Guess what piece isn't covered by the service contract? That is right, the consumable top plate that used to not be consumable. So in summary, this instrument provides more data than DLS a since each particle is counted and sized, but that also means every bit of artifact and background is counted and sized, leading to user experience needing to be higher than some instruments. I think the biggest downfall is the overall and sustained cost that seems to be designed into this instrument. The instrument is quite expensive upfront, but immediately seems to need service contracts and new channels for you to maintain the instrument."
Manufacturer's ResponseThanks you for your review. I am sorry that our service has not met expectations and I will engage with our service team about this. With regards to the Flow Cell, this was developed from user feedback of not wanting to disassemble the sample chamber for cleaning between measurements. The flow cell is a semi-consumable item and with typical use should have a lifetime of 3-6 months, otherwise should be changed annually. The Low Volume Flow Cell is compatible with our Sample Assistant autosampler, and we have reports from customers that they are using it almost 24-7 with the same flow cell for over 6 months. It is important to flush the flow cell thoroughly with water and that the gasket is allowed to air dry removed from the laser module at the end of the day or when not in use. It is possible to measure mixtures of different refractive index, but this is a more challenging sample type for the technology and requires more practice. When considering any contribution from a buffer, the sa
Member since: 2019
Organization: Fujifilm Diosynth Biotechnologies
Accurate and repeatable.
Application Area:Virology analytical development
"This instrument gives us the repeatable and accurate results that are needed for qualifications between AD and QC. It is user friendly, and the service team did a great job of training our team."
Manufacturer's ResponseThanks for your feedback Amanda. We are glad to hear that our instrument is giving you all satisfaction and especially the praise for our service team.
Member since: 2017
Not very efficient, would be better off with a ZetaSizer
Application Area:Particle size
"Not very easy to use or efficient. Takes too long to run a sample. Would be better off with a ZetaSizer instrument."
Manufacturer's ResponseThank you for your review and feedback. We are always striving to improve the user experience with our instrumentation. Although both use Brownian motion as the basis of the measurement, the NanoSight provides high resolution particles size and concentration data which should be available in under 10 minutes for a single sample (3 repeat 60 second measurements).
Member since: 2017
Organization: ArunA Biomedical
Good for basic concentration measurement needs.
Application Area:Concentration of Nanoparticles
"The Nanosight NS300 is relatively easy to use. However, the equipment is somewhat fragile and caution must be taken when using. Consumables must be purchased and replaced more often than expected which adds more than you would originally think to annual cost. It is challenging to get reproducible results as even the slightest change in settings can change concentration count. This machine is great for determining basic patterns and trends for comparing concentration but I would not fully believe concentration readings as exact measurements."
Member since: 2016
Organization: Binghamton University (SUNY)
It's excellent instrumentation. And it can visualize the nanoparticles.
Application Area:Size characterization of nanoparticles
"I am using this technique to measures the size of any type of nanoparticles. There are several more instrumentations which are able to analyze the size distribution. But, NanoSight can provide not only size distribution but also the number of particles i.e. concentration. Moreover, it can visualize the nanoparticles. Sometimes, the result doesn't match up with other techniques such as DLS. But, I believe this is the best NTA instrumentation."
The Malvern NanoSight NS300 Instrument provides an easy-to-use, reproducible platform for nanoparticle characterization.
The NS300 allows rapid, automated analysis of the size distribution and concentration of all types of nanoparticles from 10nm to 2000nm in diameter, depending on the instrument configuration and sample type. This unique technology utilizes the properties of both light scattering and Brownian motion in order to obtain the size distribution and concentration measurement of particles in liquid suspension.
With the ability to be supplied with interchangeable laser modules and the introduction of a motorized 6 place filter wheel means different fluorescent labels can be analyzed. Sample temperature is fully programmable through the Nanoparticle Tracking Analysis (NTA) software.
This versatile instrument offers the major benefit of a new enhanced fluorescence detection capability, providing truly holistic analysis. The 6 place fully software-controlled filter wheel means automated analysis of multiple fluorescent markers can be performed without the operator being present, freeing up valuable researcher time.
Malvern NanoSight NS300 Features: