MagNA Pure LC RNA Isolation Kit III (Tissue) by Roche Applied Science - a member of the Roche Group

Kit for the isolation of total RNA from mammalian tissue using the MagNA Pure LC Instrument

For general laboratory use. The MagNA Pure LC RNA Isolation Kit III (Tissue) is designed to isolate high-purity total RNA from flash-frozen or formalin-fixed, paraffin-embedded mammalian tissue samples using the MagNA Pure LC Instrument. Unfrozen tissue stabilized by specific reagents (like RNAlater) can also be used. The RNA purified from fresh tissue can be used directly in RT-PCR on the LightCycler^® Carousel-Based Systems, the LightCycler^® 480 System, standard thermal block cyclers, or other typical downstream applications in gene expression analysis. The quality of RNA isolated from formalin-fixed, paraffin-embedded tissue sections is suitable for relative quantification of mRNA by RT-PCR, especially on the LightCycler^® Instruments.

The purified RNA is an ideal starting material for array experiments.

Benefits

• Isolate high-quality total RNA from a broad range of different tissue sample types.
• Obtain total RNA with high integrity and purity suitable for highly sensitive RT-PCR.
• Obtain total RNA that is free of genomic DNA.

Product Description
Number of isolations/sample type:

• 192 isolations (6 × 32) from 1–10 mg flash-frozen or unfrozen, stabilized mammalian tissue
• 96 isolations (3 × 32) from 5–20 µm sections of formalin-fixed, paraffin-embedded (FFPE) mammalian tissue