MACSQuant^® Analyzer 10 Flow Cytometer by Miltenyi Biotec B.V. & Co. KG

The MACSQuant^® Analyzer 10 brings to life the versatility and power required for modern flow cytometry applications. Whether mining for rare cells, analysing the efficiency of your cell manufacturing process or investigating signaling pathways, you are equipped for the task at hand.

Truly hands-free operation
With a range of automated features, the MACSQuant Analyzer 10 lays the foundation for true automation present in all instruments belonging to the MACSQuant Family of flow cytometers.

• Automated startup, cleaning, and shutdown
• Automated compensation
• Automated sample labeling
• Automated MACS^® magnetic enrichment
• Automated calibration and quality control (QC)

Along with the MACSQuantify software complete automated assays are available:

• In-process and quality control in cellular therapy, i.e., chimeric antigen receptor (CAR) T cell manufacturing
• Toxicological assays
• Small particles/extracellular vesicle analysis
• 21 CFR Part 11 compliant option

Product Specs:

• Quantity: EA 
• Dimensions: (WxDxH* *adjustable touch screen) 669x400x394-553.5mm
• Applications: Flow Cytometry
• Excitation:
  • 405nm (violet)
  • 488nm (blue)
  • 640nm (red)
• Detection: FSC, SSC, 8 fluorescence channels
• Regulatory status: For research use only
• Sample format: 1.5/2.0/5.0/15/50 mL tubes or 24/48/96-well plates
• Measurement speed: < 25 minutes per 96-well plate (5 µL measurement volume per well) Referred value indicates the average of multiple experiments and can differ for individual sample materials. The measurement speed is determined by measuring the time between the movement of the robotic arm into the first measured well, and its movement out of the last measured well. The measurements were carried out at high flow rate in fast mode
• Maximum event rate: Up to 15.000 events/second
• Sample uptake volume: 1-450 µL
• Sample carry-over: 0.01 % Referred value indicates the average of multiple experiments and can differ for individual sample materials. For carryover, full 96-well plates were loaded with 200 μL/well of PBMC suspension at a nominal concentration of 10,000 cells/μL in every other well ("SRC-wells”). Alternating wells are loaded with an equal volume of MACSQuant® Running Buffer ("CO-wells”). The uptake volume was set to 100 μL and measured at medium flow rate in standard mode. The carryover is defined by sum (CO-singlet count)/sum(SRC-singlet count) × 100%
• Flow rate: 25, 50, or 100 µL/min or automate flow rate to maintain 500, 1000, or 2000 events/second
• Fluorescence performance: 5-decade logarithmic scale (10^-2 to 10³), displayed in lin, log, or hlog scales
• Compensation: Automated, or manual with 8x8 matrix, during or after acquisition
• Operating system: Embedded operating system
• Software: MACSQuantify, integrated acquisition and analysis software
• Data files: .mqd (proprietary file format), .fcs (2.0, 3.0, 3.1 compatible)
• Components: Build-in PC, 15.6” LCD touchscreen, Robotic uptake unit for sample acquisition, integrated MACS magnetic cell enrichment unit
• Accessories: 
  • MACS^® Chill Racks (5, 15, 50, 96)
  • Universal Reagent Rack (130-115-722)
  • MACSQuant^® Buffer Supply Station (130-101-841)
  • MACS^® MiniSampler Plus (130-105-745)
  • MACSQuant^® Columns (130-094-458)