xMAP® Technology uses color-coded microspheres to detect multiple analytes from a single aliquot of sample in a single reaction well, enabling you to generate more data while saving sample, time, and reagents. Depending on your targets and the application, there are five different microsphere types to select from to produce the best experimental results.
LumAvidin® are avidin-coupled polystyrene microspheres that have been dyed into 100 spectrally distinct sets or “regions”, allowing them to be individually identified by an xMAP instrument. In the multiplex assay environment the microspheres act as both the surface for the solution phase assay when reacted with a biotinylated ligand and the spectral identifier that the instrument detects. The open architecture of xMAP Technology enables users to build custom multiplex assays or select from a broad menu commercially available kits.
Each LumAvidin microsphere has deglycosylated avidin covalently coupled to its surface. These beads enable the user to quickly and easily design custom bead arrays, simply by reacting biotinylated target-specific ligand(s) with the LumAvidin microsphere; permanently linking the capture molecule(s) to the surface of the addressable microsphere. For a complete protocol, visit the Luminex website.
LumAvidin are ideal for coupling small molecules, like biotinylated peptides and offer: