Low Cell Number ChIP-Seq Services by Active Motif
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Standard ChIP-Seq protocols require at least 10,000,000 cells to perform ChIP-Seq, a number that is unattainable for many primary cell types. As a result, for some cell types, one of the major obstacles preventing researchers from generating quality ChIP-Seq data is the limited cell numbers available for those cell systems. Until now.
Active Motif’s Epigenetic Services group has made significant improvements to our ChIP-Seq protocols and pipeline, and as a result, we have reduced starting material requirements by 1,000 fold! Our team can now generate genome-wide binding profiles using as few as 10,000 cells.
The Low Cell Number ChIP-Seq Service includes:
- Customers submit cell pellets or small frozen tissues
- Fix samples, prepare chromatin and sonicate
- Perform ChIP with a ChIP-validated antibody
- Construct ChIP-Seq libraries
- Perform Next-Gen sequencing
- Analyze the data and deliver it to the customer
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