Status:
Expert ReviewerMember since: 2020
Organization: Montana State University
Reliable product
Application Area: Molecular Cloning
"Easy to use and high efficiency. Reliable product and does the job."
Status:
ReviewerMember since: 2019
Organization: The University Of Nottingham
Time saver!!
Application Area:Molecular cloning into synthetic bacteria
"When time isn't on my side in the lab, this ligase is a life saver. After an easy reaction setup and only a few minutes I have a high proportion of ligated fragments, for which I can transform into my E.coli. Meaning in a day I can have my recombinant plasmids constructed and in my bacteria, opposed to 2 days with other standard ligases."
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended inserts in as little as 15 minutes).
Rapid ligation is based on the combination of T4 DNA Ligase with a unique 2X Rapid Ligation Buffer to speed up and streamline your cloning experiments. The LigaFast™ System is designed to eliminate any further purification prior to transformation of ligated DNA. The specially formulated 2X Rapid Ligation Buffer requires no additional ATP or Mg2+ addition prior to use.
LigaFast™ Rapid DNA Ligation System Features & Benefits: