iScript RT-qPCR sample preparation reagent provides a simple and rapid procedure (<10 min) for isolating total RNA from cultured cells to use for RT-qPCR.
This reagent delivers efficient cell lysis, RNA stabilization, and removal of genomic DNA for sensitive qPCR without traditional RNA sample preparation.
iScript™ RT-qPCR Sample Preparation Reagent (170-8898) Key features and benefits include:
Have ease-of-use and simplicity — one-buffer formulation contains all of the necessary components to perform cell lysis, RNA stabilization, and genomic DNA removal in 5-10 minutes
Benefit from superior compatibility with qPCR directly from cells — sensitive detection of high-, medium-, and low-copy gene targets directly from cell lysates using real-time qPCR when combined with iScript reverse transcription and qPCR reagents
Maximize data from very few cells — reagent enables multiplex real-time detection of up to 4 targets from as few as 10 cells
Extend isolated RNA shelf-life — isolated RNA is stable up to 6 months when frozen
Use with different cell types — compatible with both suspension and adherent cell cultures