Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific

Manufacturer Thermo Fisher Scientific | View All Models  |  Available Worldwide
4.2
/
5.0
  |  10 reviews
Introducing the versatile reagent with the power to transfect your most difficult cells.


Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product image
Invitrogen™ Lipofectamine® 3000 Transfection Reagent

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Average Rating: 4.2
10 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Rutgers University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results.
Rating: 5.0

  • Application Area: Pituitary cancer cells transfection

"Easy to use and worked well on our primary cells from rats tumor tissues."

Review date: 17 May 2021 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent (0.75 mL)
  • Status:

    Reviewer

  • Member since: 2018

  • Organization: JMI



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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First choice for transfection.
Rating: 4.7

  • Application Area:In vitro

"It is a must for hard-to-transfect cells with more than 80% efficiency."

Review date: 02 Feb 2021 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UCLA



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great efficiency and works consistently.
Rating: 5.0

  • Application Area:Transfection into mammalian cells

"We use Lipofectamine 3000 to transfect plasmids into both human and mouse cells and receive at least 80-100% efficiency consistently."

Review date: 17 Apr 2020 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Reviewer

  • Member since: 2020

  • Organization: Concordia University



  • Ease of use
    4 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Rating: 3.3

  • Application Area:Transfection efficiency

"It would be great if there were more tips & tricks available for optimizing efficiency. Also, it is expensive, but it's still probably the best option."

Review date: 28 Jan 2020 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2018

  • Organization: University of Chicago



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    2 out of 5
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Reliable transfection of siRNA and plasmids.
Rating: 3.7

  • Application Area:Over-expression of proteins

"Highly effective product. Cost is high and could be more economical since need multiple replicates to perform for publication."

Review date: 17 Jan 2019 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2018

  • Organization: University of Chicago



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Lipofectamine 3000 provides excellent plasmid over-expression, sans cell toxicity.
Rating: 4.3

  • Application Area:Over-expression of protein

"Lipofectamine 3000 provided gentle but efficient transfection in several line of interest to our lab, HEI-OC1 mouse cochlear (69-fold) , NCCIT and Ntera2 (3 fold) testicular cancer lines and allowed for very nice dose response assays for cisplatin sensitvity assays. We appreciate greatly the technical service of ThermoFisher and find them an integral part of our research progress with excellent technical advise."

Review date: 05 Nov 2018 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2013

  • Organization: University of Penn



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results. Loves to use it.
Rating: 4.3

  • Application Area:Mammalian cell transfections

"It is extremely easy to use it. And it gives great results."

Review date: 04 Nov 2017 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent (1.5 mL)
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: UKl Freiburg Neurologie



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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I really enjoy using Lipofectamine reagent for cell transfections
Rating: 3.7

  • Application Area:Transfection of cells

"In our lab, we use Lipofectmamine 3000 reagent as our primary transfection reagent for a wide range of experiments. I personally have used this reagent to transfect cells for fluorescent microscopy, GST pull downs, and co-immunoprecipitation experiments. We have had success using Lipofectamine reagents in the past and the Lipofectamine 3000 appears to be the best option."

Review date: 03 Feb 2017 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: The Institute of Cancer Research



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
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Recommend to all researchers for lipid-based reagent for reverse transfection of plasmids
Rating: 3.7

  • Application Area:Transfection

"Easy protocol, good for reverse transfection with not much toxicity to cells"

Review date: 23 Jun 2016 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: University of Miami



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Good for regular cell lines
Rating: 4.0

  • Application Area:for cell transfection

"Very effective for easy to transfected attached cell lines, not so on hard to transfected ones."

Review date: 10 Feb 2016 | Invitrogen™ Lipofectamine® 3000 Transfection Reagent

Lipofectamine® 3000 Transfection Reagent leverages our most advanced lipid nanoparticle technology to provide superior transfection performance with improved application outcomes and reproducible results.

This reagent delivers superior transfection efficiency and improved cell viability for the widest range of hard-to-transfect and common cells (e.g., HEK293, HeLa).

Successfully transfect the widest variety of biologically relevant cell types with a reagent that offers:

  • Superior performance—our highest efficiency reagent for difficult-to-transfect cells.
  • Improved cell viability—gentle on your cells, with low toxicity.
  • Versatile—one reagent for DNA, RNA, and co-transfection.

 

Lipofectamine® 3000 reagent is designed to efficiently transfect difficult-to-transfect cells, yielding superior transfection performance across the broadest array of cell types. Successfully transfected cell types include:

 

Fibroblast (3T3, COS-7) Myoblast (C2C12, L6 CRL-1458)
Hepatocyte (HepG2, HuH7) Erythroleukemic cell (K562)
Breast carcinoma (MCF7, Hs578T) Prostate carcinoma (LNCap)
Lung carcinoma (A549, NCI-H460) Osteosarcoma (U-2 OS, Saos-2)
Colon carcinoma (Caco2, SW480) Pancreatic carcinoma (PANC-1)
Skin melanoma (SK-MEL-28)  

Product Models Information


Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product thumbnail

Invitrogen™ Lipofectamine® 3000 Transfection Reagent (0.1 mL)

L3000-001




Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product thumbnail

Invitrogen™ Lipofectamine® 3000 Transfection Reagent (0.75 mL)

L3000-008  |

(1)




Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product thumbnail

Invitrogen™ Lipofectamine® 3000 Transfection Reagent (1.5 mL)

L3000-015  |

(1)




Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product thumbnail

Invitrogen™ Lipofectamine® 3000 Transfection Reagent (15 mL)

L3000-150




Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product thumbnail

Invitrogen™ Lipofectamine® 3000 Transfection Reagent (5 x 1.5 mL)

L3000-075



Product Overview

Invitrogen™ Lipofectamine® 3000 Transfection Reagent by Thermo Fisher Scientific product image

Invitrogen™ Lipofectamine® 3000 Transfection Reagent

Manufacturer Thermo Fisher Scientific  |  Available Worldwide

4.3 / 5.0 | 10 reviews