iQue^®3 by Sartorius Group

"User friendly software and easy hands-on operation system. The speed of finishing screening a 384 well plate is outstanding, and the result is very accurate. The carry over from well to well sample is minimized. Maintenance is easy."

"High throughput screening (389 well libraries). Low carry over. Highly reproducible, low sample amount, intergrateable robots and incubators."

"I have used the Sartorius iQue screener since 2015 and performed number of different experiments with the instrument over the years. These experiments have included optimization of a multi-color immunophenotypic screening of primary cancer cells against a focused library of 40 compounds. In my institute, we initially used iQue HD, which has two lasers, but quite soon started using the iQue Plus. The first generation instrument (iQue HD) had lots of issues and was often shipped back for repairs. Quite recently the HD was discontinued. The newer version is much more robust and besides occasional clogs in the probe and tubing, the machine is working perfectly. Moreover, the Forecyt software has constantly evolved and keeps getting better. I especially like the intuitiveness of the software interface. Regardless, the the software is still relatively young and has some drawbacks regarding data visualization, which I'm sure will improve over the next couple of years."

"IntelliCyt's is an easy to use flow cytometer with user-friendly software. It allows a rapid and high throughput assessment of the uptake of fluorophores in 96 well and 384 well formats."

"It is a great time-saving instrument. "

"Great possibility to analyse cells togehter with their secreted proteins."

"High quality and great accuracy. Ideal for high throughput screening"

"The iQue Screener PLUS has become an invaluable part of our work flow by allowing us to quickly test many parameters - cytokine release, phenotype, functional killing, etc. - and compare the results of many samples with ease."

"The iQue Screener PLUS instrument is a great instrument to use. I am not an expert in flow cytometry but you don't need to be as it is very user friendly, especially if you use the ready-to-use kits from Intellicyt. I would recommend the instrument to any lab who needs a high-throughput flow cytometer."

"The instrument is easy to use and maintance is really easy and time-efficient. You can measure 384 well plates very fast, enabling high-throughput. Drawback is that the instrument is really a screener. In detail analysis of immune cell subpopulation is hard (but this is ok since it is meant to be a screener). The software is good however there is still room for improvement: e.g. it would be nice to gate out dead cells for compensation. Furthermore it would be nice to make batch analysis, so that you make an analysis for one sample and then say that it should be amplied to all samples."

"Useful instrument for complete assay and comprehensive data for analytical samples in our laboratory"

"I find the iQue intuitive and easy to use. I'm especially appreciative of the iQue's speed in comparison to other cytometers in our lab. Routine maintenance for the cytometer is simple and quick We have limited instances when I need to contact service, but when I do, efforts are promptly taken to help me address the issue or send us help"

"The flow cytometer itself is easy to use. However, we have clogging issues often due to samples sitting in the plate for a while, even though with constant shaking mode on. It will be better if the design can add a sample “mixing” step as BD machine does"

"Great instrument. Especially ForeCyt Software is amazing to use it."

"I just begun but my first impression is great about the speed and robustness of the device!"

"Running the instrument with ForeCyt is very easy, but the software analysis could be more simplified and intuitive. I never received a reference manual (hardcopy or .pdf) which is problematic when teaching users in a core facility to do analysis. Maintenance of the instrument is fairly simple, but the reagents are excessively expensive, especially for an academic budget. The speed and efficiency of this instrument is unmatched, but I'm not overly impressed with the quality of multi-parameter flow data output. The co-linear lasers and shared detectors make it difficult to design panels above 4-5 colors for most of the work we've done so far. Common combinations of fluors typically run on our traditional flow cytometers with relatively low amounts of spectral overlap tend to require unusually high amounts compensation on the iQue Screener Plus. The resulting spread in the data can make population resolution difficult. Some users have opted to go back to the standard flow cytometers for better data quality. We've found this instrument is best suited for simple, low-parameter panels with suspension cells. Efficiently and consistently processing sticky or adherent cells into single-cell suspensions has also been a bit of an issue causing clogging and poor data quality. It's not an easy procedure when working from plates. We've gone through more than our fair share of sample probes, which are expensive."

"This is a very easy to use, high throughput flow cytometer. I love that you can easily hook it up to a robotic arm and run all day fully automated. It also has a very convenient shutdown feature that can be automatically run at the end of your run."

"The ability to quickly screen 3000+ wells (antibodies) is a real game changer for antibody screening and development. A traditional FACS platform would require hours of set-up coupled with many hours of reading samples and still be limited in total samples screened. The software could be better tuned for screening purposes by including the ability to build panoramas across multiple "experiments, and linking samples across plates. These functions would allow full data analysis inside forcyte since now it is easier to export raw MFIs and build custom data tables in programs such as JMP or even excel."

"Ease of use. Fast acquisition. Analysis is straight forward."