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Member since: 2017
Easy to use
Application Area: Mutant deletion cloning
"Makes design and workload much easier. Really like the online tool."
Member since: 2016
Organization: University of Buenos Aires
Great cloning strategy
Application Area:Molecular Biology
"This kit turns out to be very easy to use. It saved the cloning of a difficult and long cDNA that we needed and couldn't clone with regular methods."
Member since: 2016
Organization: Gurdon Institute, Cambridge University.
Great results with the In-fusion cloning kit
Application Area:Plasmid cloning
"It was very simple and effective to use. It’s easy to design and clone. It works like a magic mixture. "
Member since: 2009
Organization: University of Milan Bicocca
"This kit is perfect for cloning larger fragment up to 4kb in first attempt. I am using this kit from last year and it’s perfect for cloning larger construct. The sensitivity is amazing and efficiency of positive clones is above 70%. I highly recommend because of its capability and sensitivity, saved lot of time as compared to normal cloning and decreases repetitive experiment as indirectly save cost as well. When I started using it was new to me as I failed with other cloning product at last I came up with this kit and worked very well since. "
Member since: 2015
Organization: University Freiburg
Application Area:Gateway-cloning of BCR constructs
"Cloning on the principle of a fire-and-forget system. Fast and easy application in combination with efficiencies of <90% positive clones. Worth to change.... "
Member since: 2008
Organization: UT Health Science Center
"The best for cloning large sequence inserts."
SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing—Single-Cell Transcriptome Analysis with Ultimate Sensitivity
The SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing is the fourth-generation of SMARTer Ultra Low kits, and our most sensitive mRNA-seq kit for single cells and ultra-low inputs. This kit takes SMART (Switching Mechanism at 5’ End of RNA Template) technology to the next level by incorporating and improving upon the SMART-Seq2 method and locked nucleic acid (LNA) technology. With these additions—referred to as "SMARTer-Seq® chemistry"—this kit is able to outperform both the SMART-seq2 method and previous generations of SMARTer kits by identifying the greatest number of transcripts. The Clontech tradition of continuous innovation extends to this kit by providing direct cDNA synthesis from as few as 1–1,000 intact cells (or as little as 10 pg–10 ng of total RNA), high reproducibility, even gene body coverage, and excellent representation of GC-rich transcripts. The full-length cDNA libraries from this kit are compatible with both Illumina® and Ion Torrent sequencing platforms.
The core SMART technology in this kit is an immensely powerful tool for studying the full transcriptome, including splice junctions and alternative splicing events in cells, and provides full-length gene coverage. Oligo(dT) priming is used to specifically amplify mRNA from either high-quality total RNA (RIN >8) or intact cells. LNA technology improves the efficiency of template switching, which increases the number of genes identified to higher levels than any other SMARTer Ultra Low kit for mRNA-seq.
Information about prior SMARTer Ultra Low RNA kits can be found here