HSV-2 IgM by DRG International Inc.

Manufacturer DRG International Inc.  |   Model: EIA-1794  |  Available Worldwide
High Quality Assays with Reproducible and Reliable Results


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HSV-2 IgM
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An enzyme immunoassay for the detection of IgM antibodies to herpes simplex virus type 2 (HSV-2) in serum. Herpes Simplex Virus is a common pathogen and its primary infection is usually asymptomatic.  There are two immunologically distinct types of HSV: Type 1 and Type 2.  HSV-1 isgenerally associated  with  oral  infection  and lesions  above  the  waist,  and HSV-2 is associated with genital infections and lesions below the waist. Clinical cases primarily are (1) eczema herpeticum with eczematous skin changes with numerous lesions, (2) gingivo-stomatitis, and (3) herpes sepsis, almost only found in premature newborn infants. The BioCheck HSV-2 IgM ELISA is an accurate serologic method to detect HSV-2 IgM specific-antibody in serum samples.Purified HSV-2 antigen is coated on the surface of microwells.  Diluted patient serum is added to the wells, and the HSV-2 IgM- specific antibody, if present, binds to the antigen.  All unbound materials are washed away.  HRP-conjugate is added, which binds to the antibody-antigen complex. Excess HRP-conjugate is washed off and a solution of TMB Reagent is added.  The enzyme conjugate catalytic reaction is stopped at a specific time. The intensity of the color generated is proportional to the amount of HSV-2 IgM-  specific antibody in the sample.  The results are read by a microwell reader compared in a parallel manner with calibrator and controls.