Green Cathepsin B Assay by Immunochemistry Technologies LLC

4.3
/
5.0
  |  4 reviews

ICT’s Green Cathepsin B Assay allows researchers to detect and monitor intracellular cathepsin activity over time in vitro using live whole cells.  This in vitro assay employs the quenched substrate Rhodamine 110-(RR)2, which fluoresces green upon cleavage by active cathepsin enzymes. Analyze samples using flow cytometry or fluorescence microscopy.


Green Cathepsin B Assay by Immunochemistry Technologies LLC product image
Green Cathepsin B Assay

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Average Rating: 4.3
4 Scientists have reviewed this product

4 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2018

  • Organization: Public Welfare Organization



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Very effective instrument
Rating: 3.7

  • Application Area: Europe and America

"This product is good and easy to use. It also give high quality results"

Review date: 14 Mar 2019 | Green Cathepsin B Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Azire Integrated Healt Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Perfect product, I recommend this for other scientists.
Rating: 5.0

  • Application Area:Daily use

"An important tool for studying enzyme kinetics and screening small molecular inhibitors used for discovering drugs. The Green Cathepsin B Assay permits scientists to discover and keep under surveillance intracellular cathepsin activity over some period in vitro with the use of whole live cells. This instrument is easy and straightforward, one can adapt it to a 96-well plate assays."

Review date: 14 Mar 2019 | Green Cathepsin B Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: St.marys College Of Sciences



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
This product is specially designed for the experimental studies.
Rating: 4.3

  • Application Area:To enquiry the activities to of cathespian inside the cell

"Yes I recommend it to others because it is easy to use .. And it can also use to check the activity and different reactions carried out during the whole process."

Review date: 14 Mar 2019 | Green Cathepsin B Assay
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Peshawar University



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Good quality product. Like it.
Rating: 4.0

  • Application Area:Used once a day

"Very effective for immune system. High quality product. Easy to use and useful for anyone."

Review date: 18 Feb 2019 | Green Cathepsin B Assay

Cathepsins are a group of protease enzymes typically found in the lysosome. Cathepsin B plays important roles in many cellular functions (like intracellular protein degradation, antigen processing, etc.). Elevated cathepsin activity is associated with many disease states such as cancer, Alzheimer’s disease, and so on.

The Green Cathepsin B Assay contains Rhodamine 110 Cathepsin B Substrate, which is a non-cytotoxic and cell membrane permeant substrate that fluoresces green upon cleavage by active cathepsins. To use Rhodamine 110-(RR)2, add the substrate directly to the cell culture medium, incubate, and analyze. Because the substrate is cell permeant, it easily penetrates the cell membrane and the membranes of the internal cellular organelles – no lysis or permeabilization steps are required. If active cathepsin enzymes are present, the quenched Rhodamine 110-(RR)2 substrate is cleaved, resulting in an increase in green fluorescence signal. Samples can be analyzed by flow cytometry or fluorescence microscopy. Hoechst 33342 is included in the kit to label nuclei.