GlycoWorks RapiFluor-MS N-Glycan Kit by Waters

Manufacturer Waters  |  Available Worldwide
4.5
/
5.0
  |  46 reviews

Reimagine Released N-Glycan Analysis.


GlycoWorks RapiFluor-MS N-Glycan Kit by Waters product image
GlycoWorks RapiFluor-MS N-Glycan Kit
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Average Rating: 4.5
46 Scientists have reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
4 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2015

  • Organization: Regeneron Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Very good product for N-linked glycan analysis by HILIC-FLR-MS
Rating: 4.7

  • Application Area: N-linked glycan analysis for therapeutic protein characterization

"Ease to use, strong FLR and MS signal for glycan ID and quantification."

Review date: 27 Jun 2017 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: merckserono



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
Easy and high throughtput kit, but it can not be applied as it is for all the proteins
Rating: 3.7

  • Application Area:Glycoprotein characterization and glycoprofiles monitoring

"The kit RapiFluor is an amazing solution for easily monitoring glycoprotein. As it is supplied, it is easy, quick and truly high throughput, as the small amount of sample is necessary. All the above combined with high fluorescence and MS signals. It is the best solution for monitoring the glycoprofiles batch to batch, with the support of MS info as provided by QDA detector. The sample preparation works well for a simple mix of glycans but it has to be optimized in case of complex protein (not antibody as IgG1) with big and very complex glycans and/or high sialylated glycans. In these cases the complete glycan release and the total recovery can be not easy. The usually hard identification of small peaks, despite the amount of starting protein is less, could be easier than in the past because of the high intensity of MS signals plus the GU values for RapidFluor in UNIFY platform."

Review date: 24 Feb 2017 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Abzena



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Really good, by far better than anything else available on the market at the moment!
Rating: 5.0

  • Application Area:Glycoprofiling

"Amazing product! Really simplifies the work flow (you can process 24 samples in less than half a day), extremely high sensitivity and resolution by HILIC, MS compatible - for precise assignment of glycan peaks. Also - the final sample volume of 400uL gives the flexibility of measuring duplicates/triplicates by both HILIC and MS. It cannot be compared with 2-AB labelling, millions of times better! The price is higher than for 2-AB labelling, but the overall benefits for this product are incomparable."

Review date: 27 Sep 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The University of Manchester



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very clear results and easy to use
Rating: 5.0

  • Application Area:Analyze biologics and new glycoenzymes

"The kit is very easy to use, especially following the 2-day training course Waters provided. The results are very clear and reproducible and considering all of the kit components it is very comparable with existing labels."

Review date: 02 Sep 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2013

  • Organization: Arven Medicine Company



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Thanks Waters
Rating: 4.3

  • Application Area:Glycosylation profile of mAbs.

"The RapiFluor-MS N-Glycan Kit is very useful for the analyst because it has very easy preparation steps. The results which are obtained are very robust."

Review date: 24 Aug 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: novartis



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great MS sensitivity.
Rating: 5.0

  • Application Area:Glycan analysis

"Clearness of the protocol and speed of the reaction. Data analysis with UNIFY could be better as database is not complete and dextran ladder is used but having the standard glycan with the rapid fluo label could help for difficult glycan. "

Review date: 17 Aug 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Hospira Healthcare ( A Pfizer Company)



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Rapifluor Kit is amazing !!!!
Rating: 5.0

  • Application Area:N-Linked glycan analysis

"My key area of interest has always been Glycobiology and I have been involved in glycan analysis since 2007. During my early days of sample preparation, it was a nightmare with regard to post labeling clean-up steps using cartridges. Life was made easy when i started using the µHILIC plates in 2014 . With the introduction of Rapifluor kit glycan analysis was never so amazing. Not just the high-throughput sample prep but also the FLR sensitivity is great especially for the low abundant glycoforms . Truly this kit can change the whole approach of Glycan analysis. The availability of the commercial Rapifluor standards including high mannose is really useful.Thanks to Waters for the marvelous innovation !!!!"

Review date: 29 Jul 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Glycotope



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Great results after certain effort on optimization
Rating: 4.7

  • Application Area:Glyoproteins

"The product offers a fast and easy sample preparation. Some steps have to be optimized for each protein individually (for optimal denaturation/deglycosylation). It delivers great MS signals. Glycans can be identified easily without interfering background/dirt signals even more valuable considering the low sample amounts. A small drawback is the low tolerance against higher buffer salt concentrations. Again this has to be evaluated for each protein separately. The manual as it is, is not applicable for all glycoproteins and needs further optimization for individual topics."

Review date: 11 Jul 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Epirus Biopharmaceuticals NL



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Very good and reliable kit.
Rating: 4.7

  • Application Area:Released Glycans on in process and DSP antibodies

"Extremely reproducible, using can be confusing, the names 'reagent 1,2,3,4'are are very confusing to me, as it is all the same. Also it should be indicated clearer if it is a box for 24 or 96 or 3 times 8. Finally, it would be good if there was a pipetting boat for all volumes to be added, especially the enzyme is a pain (1.2 microliter) when using multichannel pipet. The enzyme solution could be better 10 times diluted anyway."

Review date: 11 Jul 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Quality Assistance



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Best in class
Rating: 5.0

  • Application Area:Glycan analysis

"Very easy to use; no experience required. Yields high-sensitivity fluorescence and mass spectrometry data in a very short time. Much better than traditional 2-AB labelling. Works also well on simple quadrupole mass detectors (QDa). "

Review date: 11 Jul 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit


  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Fudan University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Quick to get exact results and easy to use
Rating: 4.7

  • Application Area:mAb (This kit helps us to get the glycan profile quickly for exiguous sample volume)

"This kit helps us to get the glycan profile quickly for exiguous sample volume; it enables high quality of results and it is easy to use for chemists, I would like to recommend to those do lots of glycan preparation work "

Review date: 30 Mar 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Pfizer



  • Ease of use
    3 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
It would be worth to try.
Rating: 3.3

  • Application Area:For vaccine candidates

"If your sample is mAb, it would be easy to use/analyze by following their protocol. But for different materials, it requires modification. It can't be straight use."

Review date: 16 Feb 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: PharmaEssentia Corp.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
good product!!
Rating: 4.7

  • Application Area:For the analysis of mAb

"The sample just needs 10 ug. The kit is easy use and high quality, I will recommend to other researchers."

Review date: 10 Feb 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Agenus, Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great product!
Rating: 5.0

  • Application Area:MS analysis of released glycans from antibodies

"The GlycoWorks RapiFluor-MS N-Glycan Kit was very easy to use and worked on the first attempt. The data was of a high quality and quantification was very comparable to traditional 2-AB labeling, but in a fraction of the time."

Review date: 10 Feb 2016 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Pall Europe



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Low titre Rapifluor Glycan analysis
Rating: 5.0

  • Application Area:Analysis within Biopharmaceuticals

"The following review highlights step alterations we made to increase titre concentrations carrying out the new RapiFluor sample preparation on our low titre Glycan samples. (Previously we used the 2-AB method to do Glycan analysis). Hopefully this will help anyone who has also encountered these issues with their own samples.
We carried out initial Glycan testing using the base method provided with the RapiFluor protocol and found our signals were too low after sample analysis on our Mass Spec. The main issue being that our titre values for samples were below the required specification, because of this we contacted Waters to see what could be done to improve this. The following instructions detail what we did to increase titre concentrations along the process and to therefore achieve increased signal for MS and FLR.
The best way to describe what we did is that we increased Front end loading and low dilution at the back end of the protocol.
Details:
To purify our samples we use GE Protein A spintrap columns. We always spintrap around 2mL of sample for each spintrap column, however even with low titre samples this will still not be enough to increase signal on its own. The final step of the procedure for using the columns is to Elute with 400uL buffer into two 30uL volumes of Neutralizing buffer. Instead we used 300uL of elution buffer into 45uL Neutralizing buffer, this decreasing of dilution should increase the sample titre concentration slightly more before attempting the Rapifluor protocol.
The next alteration is during Step 1 of the protocol (Rapid Deglycosylation), part 2 and 3 of this step details using water with Glycoprotein. However, with concentrations below 0.75mg/mL we used no water and used 22.8uL of the Glycoprotein. After this step follow the process to Step 3 (HILIC clean-up)
At Step 3, part 7, the protocol states to Elute with three 30uL volumes of SPE elution buffer. We did this, however, after the volumes were collected we dried the samples down by Vac centrifuge for 1 hour 30 minutes before starting part 8.
Then when we did part 8 instead of diluting with 100uL DMF and 210 ACN, we reconstituted with 9uL water, 10uL of DMF and 21uL ACN. This giving 40uL of your glycoprotein sample.
That is what we did to increase the sample concentrations throughout the protocol. The final thing we did was to inject 20uL sample volumes for analysis on the MS.
Below I've detailed the difference in signal strengths between our initial run using the protocol word for word, and then for the altered procedure.
Initial run: Sample concentration 0.25g/L TIC signal 4.38e4 FLR signal 10,000 EUx10e4
Altered run: Sample concentration 0.20g/L TIC signal 4.25e6 FLR signal 5,000,000 EUx10e4
The instrument was optimized to deal with this method analysis.
The advantage of this method is that it is very quick to use, even with the Vac drying stage (it still beats the 3-5 day 2AB procedure formally used).
The profiles given were very reliable and reproducible to the reactor pattern given using our previous method.
"

Review date: 08 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Seattle Genetics



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
RapiFluor-MS has provided us with unprecedented characterization of N-glycosylation
Rating: 4.0

  • Application Area:Characterization of monoclonal antibody and antibody-drug conjugate N-glycosylation

"RapiFluor-MS has provided the ability to characterize N-glycans with greater precision and confidence. It has also enabled the identification of previously unknown glycan structures and modifications, thanks to its compatibility with MS technology. Novel approaches such as RapiFluor are sometimes met with apprehension, due to lack of experience with the technology. However, RapiFluor-MS has demonstrated its utility in a number of application areas with confidence and experience continuing to grow on a weekly basis. Adoption of novel labelling technology can require extensive evaluation and comparison to legacy workflows. Information on how RapiFluor-MS compares to traditional reductive amination (2AA, 2AB, and APTS) as well as alternative separation techniques such as CE-LIF would further support RapiFluor-MS as complementary approach for glycan characterization. "

Review date: 08 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: UCB Pharma



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Overall this is a great product to produce robust data, using minimum material, in a short time for few samples.
Rating: 4.0

  • Application Area:Monoclonal antibody glycosylation profiling

"This product enables to prepare labeled glycans within one day with a minimum of initial material for a decent signal. The quality and reproducibility of the results are satisfying and align with previous classical digesting/labeling kit. The kit is easy to use and the procedure well described, but require some equipment such as a negative pressure manifold etc. On the other hand, the different tubes provided in the kit are not very suitable for the volumes pipetted. The after sales care and follow up is great and the questions asked are always answered in a short time. Finally, the value for money is decent, but the kit is of course slightly more expensive than previous kit. The gain of time is indisputable for few samples but decrease for a full 96 well plate "

Review date: 08 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: UCB Pharma



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
Overall this is a great product to produce robust data, using minimum material, in a short time for few samples.
Rating: 4.0

  • Application Area:Monoclonal antibody glycosylation profiling

"This product enables to prepare labeled glycans within one day with a minimum of initial material for a decent signal. The quality and reproducibility of the results are satisfying and align with previous classical digesting/labeling kit. The kit is easy to use and the procedure well described, but require some equipment such as a negative pressure manifold etc. On the other hand, the different tubes provided in the kit are not very suitable for the volumes pipetted. The after sales care and follow up is great and the questions asked are always answered in a short time. Finally, the value for money is decent, but the kit is of course slightly more expensive than previous kit. The gain of time is indisputable for few samples but decrease for a full 96 well plate "

Review date: 04 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Dr. Reddy's Labs Ltd



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great kit, would be using henceforth for all our characterization experiments
Rating: 5.0

  • Application Area:Characterization of biotherapeutics

"It is very fast, simple, highly sensitive in FLR and more exciting is about its MS signal sensitivity with which we could identify so far unannotated glycans"

Review date: 04 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: BMS



  • Ease of use
    3 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Overall good kit with room for improvement
Rating: 4.0

  • Application Area:glycan analysis of biologics

"The process is very simple; however, the kits lack of flexibility in terms of number of samples need to be analyzed (need to at least use 8 wells). When a large number of samples need to be run, sample preparation time will increase significantly. The cleaning step using vacuum manifold is tricky sometimes to obtain good recovery. The fluorescent signal is excellent and glycan separation is very good which we really liked. The mass spec signal acquired with QDA detector however is somewhat weak and hopefully can be improved. "

Review date: 04 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Medimmune Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
This kit changes everything when running glycans profiling
Rating: 4.7

  • Application Area:Glycosylation

"This kit is amazing. Not only the data I got was highly reproducible but the kit makes everything high throughput. You can run a 24 or 96 samples in less than a day. The average glycans profile can take up to 72 hours in sample prep time. GlycoWorks RapiFluor gets the job done in 2hours. It is also MS friendly. You simply can't get any better than that. "

Review date: 03 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: Analytical & Process Chemistry



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Highly recommend
Rating: 5.0

  • Application Area:Peptide/protein analysis

"Simple to use great support, Excellent value for money."

Review date: 03 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Regeneron Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
great MS and MSMS results
Rating: 4.3

  • Application Area:N-linked glycan analysis from antibody

"it is easy to use with great MS signal for the glycan structure identification."

Review date: 03 Dec 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: CiplaBioTec Pvt Ltd



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great Kit, Should be use for N Glycan Analysis
Rating: 5.0

  • Application Area:N Glycan analysis of IPQC and Final DS and DP samples

"Kit is highly effective, easy to use, saves lots of time and troubles as compared to previous available options of glycan analysis. "

Review date: 30 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Eurofins Lancaster Laboratories



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Great sensitivity, fast workflow
Rating: 5.0

  • Application Area:N-glycan profiling

"This product is fundamentally changing glycan analysis. It provides fast and effective sample preparation, convenient labeling and clean-up and reliable results. It made glycan analysis finally user-friendly. It also provides enhanced fluorescence and mass spectrometry sensitivity the same time. "

Review date: 30 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Medimmune



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Improved MS sensitivity, great results
Rating: 5.0

  • Application Area:Analyze glycans from therapeutic antibodies and proteins

"I start with far less protein than I use to start with and I get comparable results. The protocol is easy to follow and quick to run. Waters respond promptly to issues and they are effective at resolving them. It represents value for money because if you do not need to run 96 samples, you can buy a kit to run 24 samples. Super!"

Review date: 30 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: DTU



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Much improved over the old 2-AB protocol
Rating: 4.3

  • Application Area:N-Glycan analysis of mammalian and fungal glycoproteins

"Much more sensitive with fluorescence and also more sensitivity with MS. Previous 2-AB protocol took ~7 hours this can be done in ~2,5 hours"

Review date: 30 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Health Canada



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Easy to use kit for N-glycan analysis.
Rating: 4.3

  • Application Area:glycan analysis - monoclonal antibodies

"Easy to use, for quick analysis of N-glycans. Labelled glycans can be detected by Mass Spectrometry operating in positive ion mode. Reproducible results."

Review date: 27 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2014

  • Organization: Symphogen



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Cheaper kit, higher throughput, MS compatible
Rating: 4.3

  • Application Area:Protein glycosylation profile

"The RapiFlour-MS kit from Waters has improved the workflow on how we assess the glycosylation pattern of our protein based products. The kit is fast and robust allowing for MS quantifiable results, hence strengthen our confidence in our analytical results. This kit has given us a confidence that we move the kit into a validated environment with the intended purpose to use the results as a QC release test. Further, the kit allows for analyzing smaller lots of samples, compared to older kit that forces you to analyze 96 samples or waste the rest of the kit, thus decreasing the price of the individual analysis. "

Review date: 27 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: MSD



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great and fast product. Very robust!
Rating: 4.3

  • Application Area:Antibodies

"The rapiFluor kit is an ideal method for easy and quick measurement of glycoproteins. Because of the speed of the method, this kit is ideal for labs that have a large amount of samples."

Review date: 27 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: DSM Biotechnology Center



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Easy, fast and good MS sensitivity!
Rating: 4.3

  • Application Area:Analyzing N-glycans

"We have tried the GlycoWorks RapiFluor-MS N-glycan kit for some of our products and I was really pleased with the result. Although it was the first time we tried the kit, it really turned out to be very quick and easy to use. What especially was amazing was the similar peak profiles and sensitivity we obtained when comparing fluorescence detection and MS. This made us to decide that in the future we do not need fluorescence and only use MS for both relative quantification and identification."

Review date: 27 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Development



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
N-Glycan analysis has become a breeze with this new RapiFluor kit!
Rating: 4.7

  • Application Area:Analyze glycans

"N-Glycan analysis has become a breeze with this new RapiFluor kit! Quite a bit of optimization was needed for our specific needs, but upon completion, the kit has worked wonders for us. We have used two lots of kits and they both have worked well. I like the option to buy a smaller kit to reduce our waste."

Review date: 26 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Bayer Health Care



  • Ease of use
    4 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Very nice results!
Rating: 4.0

  • Application Area:glycan profile for the recombinant protein

"The GlycoWorks RapiFluor-MS N-Glycan Kit streamlines the workflow. It reduces complicated, time consuming sample preparation and we could have the result ready in a day including uPLC separation."

Review date: 26 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Northwestern University



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Good results so far with all standards after relatively short set-up period, looking to see what this instrument can do in the future.
Rating: 4.3

  • Application Area:Analyzing single glycosylation sites from crude bacterial lysates

"I have only run a number of standards using this instrument because this is the first time that my lab has attempted to quantify and study glycosylation using LC-MS. As someone who hasn't worked with HPLC or LC-MS before I was impressed with the after purchase support offer from Waters and the relatively simple product preparation. I will be interested to see the sensitivity that can be obtained with this instrument even in our complex lysate samples."

Review date: 26 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Regeneron Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Saved a full day of sample prep!
Rating: 5.0

  • Application Area:N-glycan analysis

"I was very impressed by the ease of using this product. It was very easy to set up and really reduced the time of our previous method by an entire day. This product will greatly improve our workflow. In addition, Waters representatives were swift in scheduling a demo and helping us every step of the way. Very happy with this purchase."

Review date: 26 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Sython BV



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
Fast sample preparation with highly reproducible high intensity results.
Rating: 5.0

  • Application Area:N-glycosylation analysis mostly on monoclonal antibodies

"This kit is easy to use and provides highly reproducible results, both with fluorescence detection and MS analysis. Moreover, sample preparation can be performed in short amount of time. "

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: BTI



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    2 out of 5
Great results where each fluorescence peak could be mass verified
Rating: 3.3

  • Application Area:analysis of N-glycans

"the product is easy to use and it has shortened sample preparation time significantly. However, each reaction is still much more expensive as compared to 2-AB labelling glycans, hence it is mainly reserved to analyze more complex samples only. "

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Takeda Pharmaceuticals



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Great instructions and good initial results!
Rating: 4.3

  • Application Area:Analyze glycans from monoclonal antibodies

"I have recently begun evaluating the RapiFluor kit. I've found the instructions and the kit contents very easy to use, and haven't had to amend the kit instructions for our own use. After an initial evaluation the glycans were well separated, further testing will confirm whether reproducible results are achieved. Overall I am happy with this kit. "

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2011

  • Organization: Gedeon Richter



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Easy to use, fast sample preparation
Rating: 4.7

  • Application Area:N-Glycan analysis

"Easy to use, fast sample preparation, it really takes only 30-40 min. Nice FL and MS signals even for the minor glycans."

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Theramyt Novobiologics Private Limited



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
Great results...will always be received for this kit
Rating: 4.7

  • Application Area:Analyze complex glycan structures in biologics

"The positives compared to other available... 1. Ease of use 2. Number of samples which can be analyzed in one go 3. Time taken to complete the analysis"

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Theramyt Novobiologics Pvt. Ltd



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
Excellent kit in terms of time and usage
Rating: 4.3

  • Application Area:Analyze glycan profile in Monoclonal antibodies

"Rapifluor kit is very much useful for characterizing the glycan profile for Novel and Biosimilar antibodies in high throughput analysis. This kit sensitivity is excellent if we are using LC/MS. We are finding difficulties in eluting the sample without a positive pressure manifold, instead if the kit also designed(plate) to use centrifuge (plate holding rotor) for washing and elution will be great added advantage. The repeatability is excellent, we found little less fluorescence signal while using only LC with fluorescence detector using this kit when compare to regular 2AB labeled glycan analysis."

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Cobra Biologics



  • Ease of use
    4 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
The kit has improved our through-put and the quality of the data.
Rating: 4.3

  • Application Area:Glycoprofiling of recombinant proteins and mAbs.

"Kit is used for glycoprofiling of recombinant proteins and mAbs. Applications are to support, clone selection, cell culture process development and characterization and release of drug substance. Review: We find the kit easy to use and highly efficient. We are experiencing problems with variability of retention times within runs when using our H-class bio system with RapiFluor-MS standard method. This in combination with insufficient peak capacity and resolving power in regions of (Man5, G1, G1F-GN) and (G2, Man6, and more) are slows down data evaluation and introduces ambiguity in peak assignments. Some of these ambiguities are inconvenient and may lead to us drawing the wrong conclusions for the experiment at hand. Apart from these comments we are very satisfied with the performance of the kit and with ease of use and through-put. "

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: MSD



  • Ease of use
    3 out of 5
    After sales service
    3 out of 5
    Value for money
    3 out of 5
Overall the kit was relatively easy to perform and delivered what is promised.
Rating: 3.0

  • Application Area:analyze glycans on mAbs

"We used the kit to analyze glycans from mAbs. What was particularly interesting for us was the claimed higher LC-MS sensitivity. Overall the kit was relatively easy to perform and delivered what is promised. "

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Gilead Sciences



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
This is a great product that I would highly recommend.
Rating: 4.7

  • Application Area:Analyzing Glycans of ours and others products for comparison between lots and brands.

"The Waters kit has been wonderful out of the box. It is very easy to use and scale, especially when compared to other brands that we have used in the past and have tested out recently. The time from prep to sample completion is extremely quick and well thought out, and the data is comparable with other tests and comes out crisp and clear."

Review date: 24 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Fujirebio Diagnostics Inc



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
The kit is exceptional in that it produces well resolved peaks, for a multitude of macromolecular samples, with ease and expediency.
Rating: 5.0

  • Application Area:Glycan characterization of cancer antibodies and antigens

"Overall the kit is exceptional. The instructions are easy to follow and everything is labeled perfectly. The requirement of additional equipment (outside of the UPLC) is minimal and should be common in most analytical laboratories set up for protein characterization, greatly reducing any additional costs. The kit is easy enough to use that you feel you aren't really doing enough for a typical digestion and analysis, however the results you get speak otherwise. The profiles are well resolved and reproducible. The sensitivity is so great that you can quantify differences in glycan species and varying amounts therein whether they be lot to lot or species to species comparisons. The kit can be purchased for small or large throughput allowing for flexibility of sample population size for development work, validation, or QC testing of a final product culminating to little or no waste of leftover reagents. Clearly a new benchmark in protein characterization has been set. "

Review date: 19 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: Repligen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
Rating: 4.7

  • Application Area:Analysis of glycan profile of monoclonal antibodies.

"The kit is amazing, very easy to use and follow the instructions. The results really reproducible, I have done this assay few times using same sample and every time I got similar results."

Review date: 17 Nov 2015 | GlycoWorks RapiFluor-MS N-Glycan Kit

Waters has developed the GlycoWorks RapiFluor-MS N-Glycan Kit. This technology enables unprecedented fluorescent and mass spectrometric performance for glycan detection while also improving the throughput of N-glycan sample preparation.

Streamlined Workflow
GlycoWorks RapiFluor-MS N-Glycan Kit reduces complicated, time consuming sample preparation.

  • Easy-to-follow protocol
  • Detailed tips and tricks provided for adaptation
  • Easily train non-glycans experts

Fast Sample Preparation
The entire process, from glycoprotein to ready-to-analyze sample, can now be completed in less than 30 minutes*. This is made possible by fast deglycosylation combined with instant labeling.

  • Increase lab efficiency
  • Quickly analyze screen samples and provide feedback in a timely manner

Unprecedented Sensitivity
RapiFluor-MS is a reagent comprised of an NHS carbamate rapid tagging group, an efficient quinoline fluorophore, and a highly basic tertiary amine for enhancing MS ionization. As an NHS carbamate, unlike alternative rapid tagging reagents, it hydrolyzes over time to generate carbon dioxide and a corresponding amine. This makes RapiFluor-MS labeling a self-quenching reaction.

Enhanced chemical properties for glycan analysis:

  • Rapid tagging
  • Efficient fluorescence
  • Enhanced ionization efficiency

Product Overview

GlycoWorks RapiFluor-MS N-Glycan Kit by Waters product image

GlycoWorks RapiFluor-MS N-Glycan Kit

Manufacturer Waters  |  Available Worldwide

4.5 / 5.0 | 46 reviews