FosmidMAX™ DNA Purification Kit by LGC, Biosearch Technologies

Manufacturer LGC, Biosearch Technologies  |   Model: FMAX046  |  Available Worldwide
5.0
/
5.0
  |  1 reviews
The FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA (Fig. 1). The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6-25 µg of fosmid DNA from 1.5 to 100 ml of a single-copy fosmid culture. Selective precipitation steps and the incorporation of Epicentre's RiboShredder™ RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications.  ... Read more


FosmidMAX™ DNA Purification Kit by LGC, Biosearch Technologies product image
FosmidMAX™ DNA Purification Kit
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Average Rating: 5.0
1 Scientist has reviewed this product

5 out of 5
Ease of use
5 out of 5
After sales service
5 out of 5
Value for money


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  • Organization: MPI-CBG



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Perfect fosmid prep
Rating: 5.0

  • Application Area: DNA isolation of fosmids

"We get perfect results using this kit for isolating fosmids out of E. coli. High yields and clean samples. We are using the fosmid DNA downstream of the isolation for bombarding it into C. elegans worms. "

Review date: 29 Feb 2016 | FosmidMAX™ DNA Purification Kit
The FosmidMAX™ DNA Purification Kit was developed for easy, reliable isolation of high-quality fosmid DNA (Fig. 1). The scalable protocol is based on a modified alkaline-lysis procedure that typically yields 0.6-25 µg of fosmid DNA from 1.5 to 100 ml of a single-copy fosmid culture. Selective precipitation steps and the incorporation of Epicentre's RiboShredder™ RNase Blend effectively remove contaminants that degrade DNA and interfere with downstream applications.
 
The FosmidMAX kit is ideal for the purification of induced fosmids produced by the CopyControl™ Fosmid Library Production Kit. The CopyControl cloning technology enables the user to grow the fosmid clones at single copy to ensure insert stability and cloning of toxic gene products and then induce the clones to high-copy number for high yields of DNA. Using the FosmidMAX Kit, up to 4 µg of fosmid DNA can be isolated from 1.5 ml of an induced clone.
 
 
Features:
  • Simple protocols isolate up to 0.6, 15, or 25 µg of fosmid DNA from 1.5-, 40-, or 100-ml cultures of a single-copy fosmid, respectively.
  • The FosmidMAX kit does not use columns or binding resins that reduce yields and shear the fosmid DNA.
  • Isolate the amount of DNA that is needed, without wasting kit reagents.
  • Ideal for isolating DNA from single-copy fosmid clones, induced CopyControl fosmid and cosmid clones.
  • No columns or toxic organic solvents.

LGC, Biosearch is the exclusive source for the Epicentre brand to scientists worldwide.

Product Overview

FosmidMAX™ DNA Purification Kit by LGC, Biosearch Technologies product image

FosmidMAX™ DNA Purification Kit

Manufacturer LGC, Biosearch Technologies  |  Available Worldwide

5.0 / 5.0 | 1 reviews