Member since: 2020
Game-changing plate system for increasing proteomics throughput.
Application Area: Peptide sample prep for proteomics
"This product is a game changer for processing many samples for proteomics analysis. Prior to use of this product, each sample had to be processed in an individual C18 column. With this plate method, we are able to increase throughput by an order of magnitude. Benchmarking showed <5% difference with the individual tube method."
The Thermo Scientific EasyPep 96 MS Sample Prep Kit provides pre-formulated reagents and a robust method for the preparation of high-quality samples for proteomic MS analysis in a high throughput format.
Features of the EasyPep 96 MS Sample Prep Kit include:
The EasyPep 96 MS Sample Prep Kit enables efficient and reproducible processing of cultured mammalian cells, tissues, and plasma for proteomic MS analysis. The kit contains pre-formulated buffers, MS-grade enzyme mix, peptide clean-up plate, and an optimized protocol to generate MS-compatible peptide samples in less than four hours. The kit is optimized to process protein sample sizes from 10 to 100 µg with a high yield of MS-ready peptides in a higher throughput format.
The sample preparation of peptides for MS analysis is complex, with numerous steps and varied protocols, resulting in variability in both quality and quantity of peptides. This kit has been designed to improve reproducibility while saving hands-on and processing time. MS sample preparation has been simplified and standardized through the addition of Universal Nuclease to reduce viscosity from nucleic acids (replacing sonication), a rapid 'one pot' reduction/alkylation solution for cysteine modification (carbamidomethylation, + 57.02), and a trypsin/Lys-C protease mix for more complete digestion. In addition, the kit includes a Peptide Clean-up plate and buffers to prepare detergent-free peptide samples for MS analysis. The optimized reagents and protocol produce high-quality peptides that are directly compatible with TMT labeling, phosphopeptide enrichment, fractionation, or are ready for MS analysis.